We narrowed to 11,540 results for: Vars;

1. Endo-APEX2

   Plasmid

   #215553

   Purpose

   Stable, inducible expression in endosome

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   EGFP-2xFYVE-APEX2-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSK-Sasg

   Plasmid

   #214350

   Purpose

   Expresses cloning backbone for SlugCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 tracrRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GluA1-scFv-Halo

   Plasmid

   #230055

   Purpose

   AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mWasabi-N1

   Plasmid

   #54765

   Purpose

   Localization: N1 Cloning Vector, Excitation: 493, Emission: 509

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Use

   Tags

   mWasabi

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

5. MS2_SC2_S_Delta

   Plasmid

   #175950

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Delta (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTW170b2

   Plasmid

   #136929

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gVI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PTPN11 in pENTR

   Plasmid

   #216604

   Purpose

   gateway cloning donor vector containing PTPN11

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   PTPN11 (PTPN11 Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GeNL(Ca2+)_60/pcDNA3

   Plasmid

   #85207

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_60
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

   Plasmid

   #200631

   Purpose

   Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pTRE3G

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECFP-DEVG-Venus

    Plasmid

    #24538

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    ECFP-DEVG-Venus
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    DEVDR mutated to DEVG

    Promoter

    CMV

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pX330A_dCas9-1x2

    Plasmid

    #63596

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST-Ki

    Plasmid

    #44563

    Purpose

    for integrative constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRPaCas9

    Plasmid

    #111819

    Purpose

    Expresses Cas9 in T. brucei (2T1) cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pECFP-IETD2x-Venus

    Plasmid

    #24536

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    ECFP-IETD2x-Venus
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCLX-UBI-mCherry

    Plasmid

    #114275

    Purpose

    Ubiquitous expression of the mCherry living color

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSETB-de4GFP

    Plasmid

    #85472

    Purpose

    Inducible expression of dual emitting pH sensitive GFP in bacterial cells

    Depositor

    S. James Remington

    Article

    Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

    Insert

    de4GFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S65T/C48S/H148C/T203C

    Promoter

    T7

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJ211_WT_low

    Plasmid

    #128848

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-EF1alpha-scFv-VP64_Blast

    Plasmid

    #192655

    Purpose

    3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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