We narrowed to 3,637 results for: golden gate

1. pKL1-424

   Plasmid

   #219412

   Purpose

   Reporter gene (3 operator) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (42-10 op)x3-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKL1-095

   Plasmid

   #219407

   Purpose

   Reporter gene (4 operators) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (13-6 op)x4-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKL1-423

   Plasmid

   #219411

   Purpose

   Reporter gene (2 operator) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (42-10 op)x2-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L0-I26 pUBQ10 GG0-GG1

   Plasmid

   #195902

   Purpose

   Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   pUBQ10 (UBQ10 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOD_C'4800EC

   Plasmid

   #176875

   Purpose

   Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTSB-FICAT_Payload-Esp3I

   Plasmid

   #179380

   Purpose

   Transposon vector prepared for Esp3I golden gate cloning of Transgene

   Depositor

   Marc Güell

   Article

   Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CSM160

   Plasmid

   #105684

   Purpose

   Plasmid containing an RFP dropout used to construct randomized PAM library

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   RFP Golden Gate dropout
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_R0040 TetR Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Sth_I-E array_GFP dropout

   Plasmid

   #178775

   Purpose

   Golden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays

   Depositor

   Chase Beisel

   Article

   Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

   Insert

   gfp dropout to generate S. thermophilus type I-E single arrays
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJo55

   Plasmid

   #159623

   Purpose

   An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

   Depositor

   Vasiliy Taranov

   Article

   Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pL0-BvCYP76AD1

    Plasmid

    #162529

    Purpose

    Beta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    CYP76AD1
    

    Use

    Puc19-derived

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

    Plasmid

    #139237

    Purpose

    mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJM454 EF1alpha EBFP2-P2A-BlastR (TUPV5)

    Plasmid

    #139234

    Purpose

    mMoClo TUPV5, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

    Plasmid

    #139233

    Purpose

    mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

    Plasmid

    #139232

    Purpose

    mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

    Plasmid

    #139231

    Purpose

    mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD882 FKBP-ZF43 (NES)

    Plasmid

    #138854

    Purpose

    Constitutive expression of FKBP-ZF43 (NES) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    FKBP-ZF43 (NES)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD881 VPR-FRB (NES)

    Plasmid

    #138853

    Purpose

    Constitutive expression of VPR-FRB (NES) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-FRB (NES)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPD880 VP64-FRB (NES)

    Plasmid

    #138852

    Purpose

    Constitutive expression of VP64-FRB (NES) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP64-FRB (NES)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPD684 VPR-FRB (no NLS)

    Plasmid

    #138851

    Purpose

    Constitutive expression of VPR-FRB (no NLS) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-FRB (no NLS)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPD683 VP64-FRB (no NLS)

    Plasmid

    #138850

    Purpose

    Constitutive expression of VP64-FRB (no NLS) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP64-FRB (no NLS)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge