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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,185 results for: golden gate

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Showing: 2541 - 2560 of 5185 results

  1. pAIP4_1UXZ

    Plasmid
    #234166
    Purpose
    Heterologous protein expression of cellulase B in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UXZ
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234166-plasmid-map-sequence-id-464132

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  7. pMVRha

    Plasmid
    #229553
    Purpose
    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.
    Depositor
    Sandy Schmidt
    Article
    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229553-plasmid-map-sequence-id-461178

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  9. PAC_E83_LOV_eGFP

    Plasmid
    #231574
    Purpose
    This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light
    Depositor
    Dominik Niopek
    Insert
    PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
    Expression
    Mammalian
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231574-plasmid-map-sequence-id-460758

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  10. MbCas12a_N1153_GPG_GR2

    Plasmid
    #231578
    Purpose
    Encodes a cortisol inducible variant of MbCas12a
    Depositor
    Dominik Niopek
    Insert
    MbCas12a with a GR2 insertion behind N1153
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231578-plasmid-map-sequence-id-459789

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  15. MultiGreen

    Plasmid Kit
    #1000000250
    Purpose
    Modular assembly of multi-gene expression vectors.
    Depositor
    Wayne Parrott
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (GreenGate)
    Available Since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. CROPseq-Puro-24xMS2

    Plasmid
    #212629
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212629-plasmid-map-sequence-id-420222

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Showing: 2541 - 2560 of 5185 results