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We narrowed to 20,448 results for: kars;

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pHSV-TK

Plasmid

#165801

Purpose

Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

HSV-TK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

Plasmid

#205269

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

Codon Optimised wtCNa

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pDsRed1-N1 RanGAP

Plasmid

#13386

Depositor

Mary Dasso

Article

Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

Insert

RanGAP (RANGAP1 Human)

Use

Tags

DsRed1

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ZoAl N-1F

Plasmid

#203763

Purpose

For expression of N-flag tagged ZoAl R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Sox9

Plasmid

#41080

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pWALIUM-intron 3x UAS attB

Plasmid

#206360

Purpose

includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

Depositor

Erdem Bangi

Article

Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-teLuc c-myc

Plasmid

#100026

Purpose

Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

teLuc, a NanoLuc mutant

Use

Tags

c-myc

Expression

Mammalian

Mutation

D19S, D85N, C164H

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAB1620 hU6-BpiI-Cas13bt3-DR

Plasmid

#176315

Purpose

hU6-BpiI-Cas13bt3-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt3 crRNA direct repeat

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-NHERF1

Plasmid

#31650

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Use

Tags

His & S tag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-2

Plasmid

#26807

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

L7V, G76S, K395R and I482L

Promoter

CMV

Available since

July 26, 2011

Availability

Academic Institutions and Nonprofits only

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pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

Plasmid

#205268

Purpose

Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac UV5

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pET24a isPETase

Plasmid

#225759

Purpose

Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

isPETase

Use

Tags

6xHis Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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SFG.CNb30_opt.IRES.eGFP

Plasmid

#22493

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)

Use

Retroviral

Tags

Expression

Mutation

L124T; K125-LA-Ins

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pC-HBH

Plasmid

#88896

Purpose

Carries phiC31 integrase attB site flanked by cHS4 insulators.

Depositor

Michele Calos

Article

Zhao et al PLoS One. 2014 Apr 29;9(4):e96279. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-V5-ccdB

Plasmid

#87064

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pWUR702

Plasmid

#53079

Purpose

Inducible TtAgo expression

Depositor

John van der Oost

Article

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Use

Tags

Strep-II tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TaGu N-1F

Plasmid

#203762

Purpose

For expression of N-flag tagged TaGu R2 protein in mammalian cells

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-shCntrl

Plasmid

#69040

Purpose

Lentiviral expression of control shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

control shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCHERRY3

Plasmid

#24659

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pC007 - pRFP constitutive expression

Plasmid

#79156

Purpose

RFP constitutively expressed

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

RFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag BRG1

Plasmid

#1957

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRG1 (SMARCA4 Human)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1 Stu2 1-590

Plasmid

#38314

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 1770 bp from STU2 ORF (STU2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pSMP-RING1_2

Plasmid

#36356

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
CMV-CytERM-mCherry-(F30-2xPepper)10

Plasmid

#129403

Purpose

CytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

CytERM-mCherry-(F30-2xPepper)10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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