We narrowed to 22,481 results for: SCR

1. sgRNA1_Tet-inducible Luciferase reporter

   Plasmid

   #64161

   Purpose

   Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for Tet-inducible Luciferase reporter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG BacMam C term 3C eGFP myc

   Plasmid

   #160685

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   His8 eGFP 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBKT7-RmMYB108

   Plasmid

   #173183

   Purpose

   Expresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.

   Depositor

   Daidi Che

   Article

   Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

   Insert

   RmMYB108
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. cPE

   Plasmid

   #183549

   Purpose

   Expresses compact Prime Editor in mammalian cells

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   SpCas9 nickase (H840A), Reverse Transcriptase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3TS-Dest_R1-R3

   Plasmid

   #140878

   Purpose

   166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ccdb/CAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBlue-loxPuro-APEX-PML_LASA

   Plasmid

   #140273

   Purpose

   a targeting plasmid for APEX-PML

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   APEX2
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   endogenous

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pARNTL.1.0-gDNA

   Plasmid

   #112480

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL

   Depositor

   Kevin White

   Insert

   ARNTL (BMAL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

   Plasmid

   #159487

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-2_PolyU(1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIC.B3.TM0851

   Plasmid

   #11530

   Depositor

   Sung-Hou Kim

   Article

   Liu et al J Mol Biol. 2005 Jul 29. 350(5):987-96.

   Insert

   heat-inducible transcriptional repressor (TM0851 T. maritima)
   

   Use

   Tags

   HisTag, TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GLN, Linear-3, PolyU(1)

    Plasmid

    #166984

    Purpose

    Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ptagBFP-LacI-NLS-VP16

    Plasmid

    #103837

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-LacI-NLS-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEG BacMam N term myc eGFP 3C

    Plasmid

    #160682

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    myc eGFP 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHEY1.1.0-gDNA

    Plasmid

    #112393

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1

    Depositor

    Kevin White

    Insert

    HEY1 (HEY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTFAP2C.1.0-gDNA

    Plasmid

    #113792

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2C

    Depositor

    Kevin White

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV Lex VP16 (F442A) HA (P#1710)

    Plasmid

    #14595

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pGEM4Z globin NB Probe

    Plasmid

    #120390

    Purpose

    Allows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probe

    Depositor

    Niels Gehring

    Article

    Voigt et al Nat Protoc. 2019 May;14(5):1603-1633. do

    Insert

    beta globin partial 3' UTR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116956

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pR-Avr

    Plasmid

    #22650

    Depositor

    Lynn Hudson

    Article

    Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

    Insert

    Myelin Transcription Factor 1 (Myt1 Rat)
    

    Use

    Cloning and rna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

21. pmMyt1

    Plasmid

    #22713

    Depositor

    Lynn Hudson

    Article

    Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

    Insert

    Myelin Transcription Factor 1 (Myt1 Mouse)
    

    Use

    Cloning vector tt/aa

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJUB2x

    Plasmid

    #208814

    Purpose

    Encodes GFP under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Two copies of JUB1 binding site within the synthetic promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    JUB1 TF-responsive promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCambia3301-AtU6-MAR1

    Plasmid

    #210757

    Purpose

    Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

    Depositor

    Daisuke Miki

    Article

    Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

    Insert

    AtU6-26 pro::MAR1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC57 mini-ZsGreen-P2A

    Plasmid

    #222648

    Purpose

    Vector for cloning gene of interest for in vitro transcription

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    ZsGreen
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    mutated stop codon prior to a P2A peptide

    Promoter

    T7

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. int>pro::GFP

    Plasmid

    #218576

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Insert genomic sequence 41-372nt to -46nt upstrea…

    Promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pro::EX1-2(intΔNAC2):GFP

    Plasmid

    #218574

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation genomic sequence 206nt, 207nt from CT to…

    Promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pro::EX1-2(intΔNAC1):GFP

    Plasmid

    #218575

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation genomic sequence 85nt, 86nt from TA to CG

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pro::EX1-2(intΔNAC3):GFP

    Plasmid

    #218573

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation genomic sequence 334nt, 335nt from AG to…

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. int>UTR::GFP

    Plasmid

    #218577

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Insert genomic sequence 41-372nt to -622nt upstre…

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pro::EX1-2(intΔMYBs):GFP

    Plasmid

    #218571

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Mutation genomic sequence 187nt, 188nt from TG to…

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pro::EX1-2(intΔ4):GFP

    Plasmid

    #218569

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Deletion genomic sequence from 254nt-343nt

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pro::EX1-2(intΔ3):GFP

    Plasmid

    #218568

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Deletion genomic sequence from 182nt-271nt

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pro::EX1-2(intΔ2):GFP

    Plasmid

    #218567

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Deletion genomic sequence from 132nt-221nt

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pro::EX1-2(intΔ1):GFP

    Plasmid

    #218566

    Purpose

    Transcriptional reporter for ARF7 promoter

    Depositor

    Anthony Bishopp

    Insert

    AT5G20730 (NPH4 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Deletion genomic sequence from 58nt-147nt

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTcI39-PRM-wasabi

    Plasmid

    #195555

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET15 PemrR-gfp biosensor

    Plasmid

    #185793

    Purpose

    The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

    Depositor

    Steven Hallam

    Article

    Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

    Insert

    pEmrR-gfp
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PemrR

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCS2-foxd4l1.1-F>E

    Plasmid

    #185508

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    F>E

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2-foxd4l1.1-A6

    Plasmid

    #185506

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    FSIENIM to AAAAAAM

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt

    Plasmid

    #166993

    Purpose

    Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(0), Hairpin = 0 nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GLY, Linear-3, PolyU(4)

    Plasmid

    #166989

    Purpose

    Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination module:PolyU(4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge