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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ333: pU6-MmeFz2_omegaRNA-entry

Plasmid

#205267

Purpose

Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDmVasa

Plasmid

#165783

Purpose

Drosohphila vasa germline promoter and 5'UTR

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vasa promoter

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_BAP_his

Plasmid

#210402

Purpose

For expression of VHH with C-terminal biotin acceptor peptide and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Use

Tags

biotin acceptor peptide-his6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his

Plasmid

#210405

Purpose

For expression of VHH with C-terminal his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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PX458 pSp Cas9 Rhino1 tagA

Plasmid

#211627

Purpose

Cas9 Rhino1 tagA

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

RHNO tagA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLIP-puro-dsRed-GFP-miRNA

Plasmid

#32704

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

emGFP-miRNA

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-I-SceI

Plasmid

#165041

Purpose

Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

I-SceI

Use

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03

Plasmid

#67137

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis eGFP tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pZ148-BhCas12b-sgRNA-scaffold

Plasmid

#122448

Purpose

Expresses sgRNA for BhCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BhCas12b-sgRNA-scaffold, CMV-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TPI-WT

Plasmid

#130697

Purpose

Expresses TPI-Wildtype NMD reporter

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-Wildtype

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-csgA

Plasmid

#89581

Purpose

Express csgA curli protein with GS linker and Histaq

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgA (csgA )

Use

Tags

3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ332: pU6-GtFz1_omegaRNA-entry

Plasmid

#205265

Purpose

Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET21c-eIF4A

Plasmid

#37230

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF1 (TIF1 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
Vh-HA-3xFRB

Plasmid

#20223

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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FRB-myc

Plasmid

#20228

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T2098L point mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCMV_dCas9_VP64

Plasmid

#49015

Purpose

encodes human-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (human-codon-optimized)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ123: pRS426-His-MBP-SpuFz1_PSP1

Plasmid

#205256

Purpose

S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-TAR Variant-1

Plasmid

#129406

Purpose

circular TAR Variant-1

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-TAR Variant-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCF1135

Plasmid

#222106

Purpose

E. coli - Veillonella shuttle plasmid with single selectable marker

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Type

Empty backbone

Use

E. coli-veillonella shuttle plasmid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHERRY10

Plasmid

#24664

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-cdc2 (716)

Plasmid

#61840

Purpose

mammalian expression of cdc2

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

cdc2 (CDK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pMJ240: pRS426-His-MBP-MmeFz2_Native

Plasmid

#205255

Purpose

S. cerevisiae expression vector for MmeFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

Plasmid

#205261

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.2-ccdB-Renilla

Plasmid

#87073

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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