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Plasmid#168489PurposeMammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.DepositorInsertPKA regulatory subunit IIα C-terminally tagged by sREACh
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mLIG3-BRCT
Plasmid#159321PurposeMammalian cell expression of mouse LIG3-BRCTDepositorInsertmLIG3-BRCT (Lig3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A
Plasmid#159131PurposeExpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationY11APromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-VprBP (N909)
Plasmid#133588PurposeExpresses human VprBP N909 mutant in mammalian cellsDepositorInsertVpr (HIV-1) binding protein (DCAF1 Human)
UseTagsHAExpressionMammalianMutationC590 mutation of human VprBP lacking the DDB1-bin…PromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-Dronpa
Plasmid#138250PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-Dronpa
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc (Legacy)
Plasmid#136437PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 FcDepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsFc region of IgG1 (a.a. Pro-217 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524)PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc-Y206S
Plasmid#136439PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524) …PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc-V242K
Plasmid#136440PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524) …PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2(732)
Plasmid#154099PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v4-Fc(IgA1)
Plasmid#145159PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 4DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgA1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-T92Q
Plasmid#145156PurposeMammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutantDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynNanobody IRES EGFP
Plasmid#136624PurposePlasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-EnhancerNb (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) eGFP SH3BP5 G4
Plasmid#129022PurposeExpresses an eGFP mRNA with the SH3BP5 G-quadruplex (G4) region in the 3' UTRDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 mSmoothened NQ4
Plasmid#126407PurposeExpression of Smoothened NQ4 in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationSmo glycosylation site NQ4PromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 mSmoothened NQ3
Plasmid#126406PurposeExpression of Smoothened NQ3 in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationSmo glycosylation site NQ3PromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-1beta
Plasmid#124140PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RPS14(2-88)
Plasmid#122298Purposeexpresses a part of RPS14 protein with a flag tagDepositorInsertRPS14 (partial) (RPS14 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 89 to 151PromoterCMVAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-HF2-BE3
Plasmid#120397PurposeExpresses HF2-BE3 in mammalian cellsDepositorInsertHF2-BE3
UseTagsExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-CαN-mPAGFP
Plasmid#114130PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only