We narrowed to 3,637 results for: golden gate

1. pEPOR1CB0071

   Plasmid

   #117559

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting RPS4A family protein {AT5G58420 } in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_RPS4A family protein {AT5G58420 } A. thaliana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOR1CB0027

   Plasmid

   #117558

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtADH gene gene in A. thaliana with 1 mismatch at PAM

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_atADH1{At1G77120) _1mismatch in PAM A. thaliana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPOR1CB0021

   Plasmid

   #117557

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPOR1CB0020

   Plasmid

   #117556

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPOR1CB0019

   Plasmid

   #117555

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEPOR1CB0018

   Plasmid

   #117554

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 (pICSL90002)

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEPOR1CB0014

   Plasmid

   #117550

   Purpose

   Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S+5'UTR OMEGA (pICH51288)

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR0SP0019

   Plasmid

   #117529

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.1 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEPOR0SP0017

   Plasmid

   #117527

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.0 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K810A, K1003A, R1060A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTU2_2b

    Plasmid

    #73011

    Purpose

    Secondary module (BpiI) site in pTU2b backbone

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Secondary module + Golden Gate pTU2b BsmBI destination site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. L0-I35 p35S-XVE-pEstradiol GG0-GG1

    Plasmid

    #195904

    Purpose

    Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    p35S-XVE-LexA-p35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAGM4723:TpCC_Urease

    Plasmid

    #85982

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Inserts

    Cas9
    Urease-targeting gRNA 1
    Urease-targeting gRNA 2
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    NLS and YFP

    Expression

    Mutation

    Promoter

    FCP and U6

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB092 FoMV-sgMgCh

    Plasmid

    #234815

    Purpose

    To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting SbMgCh
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCB094 FoMV-sgLw

    Plasmid

    #234816

    Purpose

    To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting SbLw
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGY037 BSMV-gamma-AmCyan

    Plasmid

    #234818

    Purpose

    To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICSL90002

    Plasmid

    #68261

    Purpose

    Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6-26 (Arabidopsis thaliana)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC-GoldyTALEN

    Plasmid

    #38143

    Purpose

    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

    Depositor

    Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Tags

    AcV5 and FokI homodimer

    Expression

    Mammalian

    Mutation

    Truncate at N and C terminous

    Promoter

    miniCaggs

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Daniel Carlson, Stephen Ekker

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Expression

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRAM28

    Plasmid

    #234314

    Purpose

    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

    Depositor

    James Chappell

    Article

    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

    Insert

    U1367 Targeting RAM, Barcode 2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    P. CymRC

    Available since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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