We narrowed to 15,126 results for: set

1. AAVS1_Puro_hPGK1_EGFP_Donor

   Plasmid

   #178088

   Purpose

   HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1_Puro_hPGK1_EBFP_Donor

   Plasmid

   #178089

   Purpose

   HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EBFP (T65S/F145Y)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBUE411-GGB

   Plasmid

   #198233

   Purpose

   Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

   Depositor

   Andrea Gallavotti

   Inserts

   GRF4-GIF1 chimera cassette
   pPLTP::BBM cassette
   

   Use

   CRISPR; Plant binary vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin and maize PLTP

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK1035

   Plasmid

   #8832

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

   Insert

   TVMV protease
   

   Use

   Tags

   His and MBP (cleaved)

   Expression

   Bacterial

   Mutation

   synthetic gene with codons optimized for E. coli

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT-GEM(2)

   Plasmid

   #62894

   Purpose

   Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT-GEM(1)

   Plasmid

   #62893

   Purpose

   Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg

   Plasmid

   #159927

   Purpose

   Gene targeting

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

   Insert

   ERT2CreERT2
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFvL118

   Plasmid

   #64752

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW081

   Plasmid

   #64755

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKV016

    Plasmid

    #64767

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHPK1212

    Plasmid

    #8834

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    tobacco vein mottling virus protease, catalytic domain
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_dNmeCas9

    Plasmid

    #120077

    Purpose

    dNmeCas9 with no sgRNA cassette lenti construct

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    dNmeCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3087 pBS Bcl-2-Ig

    Plasmid

    #8809

    Depositor

    Stanley Korsmeyer

    Article

    Seto et al EMBO J 1988 Jan;7(1):123-31.

    Insert

    Bcl-2-Ig (BCL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

14. S111

    Plasmid

    #174549

    Purpose

    Constitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)

    Depositor

    Bruno Correia

    Article

    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

    Insert

    BclxL and designed protein LD3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. S112

    Plasmid

    #174550

    Purpose

    Constitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)

    Depositor

    Bruno Correia

    Article

    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

    Insert

    Bcl2 and designed protein LD3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTW087

    Plasmid

    #64756

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    De Vos et al EMBO Rep. 2011 Sep 1;12(9):956-62. doi:

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. SUPERBLADE5

    Plasmid

    #134913

    Purpose

    Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

    Depositor

    Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. kanMX6-ins6

    Plasmid

    #195043

    Purpose

    pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKI-cjFOXP2-hum

    Plasmid

    #129757

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    FOXP2 (LOC100411307 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    T301N/N323S

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. natMX-ins7

    Plasmid

    #195044

    Purpose

    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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