We narrowed to 7,416 results for: 11

1. pBAD_FLAG-GFP(Y39TAG)-6His

   Plasmid

   #223510

   Purpose

   Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Insert

   GFP(Y39TAG)
   

   Tags

   6xHis and FLAG

   Expression

   Bacterial

   Mutation

   Y39TAG

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. KRAB-dSpCas9

   Plasmid

   #205248

   Purpose

   In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

   Plasmid

   #78231

   Purpose

   Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   Cas9
   

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IZ-ANXA6-mRuby3

   Plasmid

   #184892

   Purpose

   Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   ANXA6 (ANXA6 Human)
   

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   R587K

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dat - EGFP - Tol2

   Plasmid

   #85418

   Purpose

   for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

   Depositor

   Marc Ekker

   Article

   Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

   Insert

   dopamine transporter (slc6a3 Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Promoter

   dat

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pmEGFP-C1-Nir3-LNS2

   Plasmid

   #220087

   Purpose

   C-terminal PA binding domain of Nir3

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM2 (PITPNM2 Human)
   

   Tags

   mEGFP-GGGGSHM

   Expression

   Mammalian

   Mutation

   amino acids 925-1209

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1-alpha-IIb

   Plasmid

   #27288

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha IIb (ITGA2B Human)
   

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-tetO-hMYC

   Plasmid

   #20723

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   c-MYC (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Xl-VSP1 R152Q

   Plasmid

   #51881

   Purpose

   expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available Since

   May 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-TREtight-EGFP

    Plasmid

    #182967

    Purpose

    For making cell lines for titering pRV∆L-5tTA virus

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EGFP
    

    Use

    Unspecified

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIB1-CreC(N1)

    Plasmid

    #75367

    Purpose

    Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Tags

    Cre DNA recombinase, residues 106-343

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRY2(L348F)-CreN

    Plasmid

    #75368

    Purpose

    Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    Cre recombinase, residues 19-104

    Expression

    Mammalian

    Mutation

    L348F

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TALEN-CCR5L18-NtermN1

    Plasmid

    #51444

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. TALEN-CCR5R18-NtermN1

    Plasmid

    #51445

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR657

    Plasmid

    #65214

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-2 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF287_EFS-TiCas9-P2A-Puro

    Plasmid

    #211646

    Purpose

    EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Tamoxifen-inducible SpyCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human_TFAM_NoMTS_pET28

    Plasmid

    #34705

    Depositor

    David Chan

    Article

    Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

    Insert

    TFAM (TFAM Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Encodes residues 43–246, corresponding to full-le…

    Promoter

    T7

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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