We narrowed to 5,570 results for: donor

1. p15_attB(bxb)_lox

   Plasmid

   #183764

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-Grin1 KI #2

   Plasmid

   #139658

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Blasticidin-CAG-Flpe-ERT2

   Plasmid

   #68461

   Purpose

   AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   mammalian-codon-optimized Flpe recombinase fused with ERT2
   

   Use

   Synthetic Biology

   Tags

   ERT2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-trGluc

   Plasmid

   #158959

   Purpose

   DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   trGluc
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2Apuro-Stargazin-mEGFP-iLID

   Plasmid

   #178529

   Purpose

   Expresses Stargazin-mEGFP-iLID in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   Stargazin-mEGFP-iLID
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mEGFP-PKCa-C1

   Plasmid

   #112264

   Purpose

   ITRACKalpha Donor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   protein kinase C alpha (PRKCA Bovine)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   A11V (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR_attB(C31)_FRT

   Plasmid

   #183761

   Purpose

   phiC31-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS_366

   Plasmid

   #182131

   Purpose

   expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

   Depositor

   George Church

   Article

   Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

   Inserts

   retron RNA
   ec.86 RT
   CspRecT
   

   Mutation

   incorporated rifampicin-resistance-conferring don…

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

   Plasmid

   #62956

   Purpose

   Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid

    #119868

    Purpose

    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIA10.5-Clover

    Plasmid

    #214449

    Purpose

    BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    mClover
    

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

    Plasmid

    #62954

    Purpose

    Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

    Plasmid

    #62955

    Purpose

    Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCyRFP1-RhoA

    Plasmid

    #84358

    Purpose

    fusion protein of RhoA, FRET donor

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1-RhoA (RHOA Human, Synthetic)
    

    Tags

    mCyRFP1 N terminal fusion to RhoA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPP5233

    Plasmid

    #128715

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    shcM-hopM1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFETCh_KDM1A

    Plasmid

    #86260

    Purpose

    Donor vector for 3' FLAG tag of human KDM1A

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KDM1A homology arms (KDM1A Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUT_mNF-Cas13d-Triplex

    Plasmid

    #227709

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d-Triplex
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-d2i

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORANGE Rims1-GFP KI

    Plasmid

    #131494

    Purpose

    Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rims1 Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pP2A_NLS_sfGFP

    Plasmid

    #63709

    Purpose

    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    

    Use

    Just a donor dna with nanog homologous arm for kn…

    Tags

    sfGFP

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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