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MAC-SARS-CoV-2 NSP3

Plasmid

#158354

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP3 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCK082 desmaMCS:minprommCherry,exorh:mCerulean

Plasmid

#200015

Purpose

Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA11

Plasmid

#179681

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJB02

Plasmid

#111959

Purpose

Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Inserts

mEGFP
eDHFR

Tags

DHFR

Expression

Bacterial

Mutation

eDHFR;residues 2-159 and enhanced GFP with monome…

Promoter

T7

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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459 p3E-2A-nlsEGFPpA

Plasmid

#195969

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-nlsEGFP (nuclear)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pENTR223-BILF1

Plasmid

#195797

Purpose

Gateway Entry Clone of Epstein-Barr virus BILF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BILF1 (BILF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BILF2

Plasmid

#195788

Purpose

Gateway Entry Clone of Epstein-Barr virus BILF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BILF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pME-p3E-HyPer7

Plasmid

#194289

Purpose

Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

Multisite gateway entry clone

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pETCK3 TGP-E

Plasmid

#236255

Purpose

Expresses TGP-E, a thermostable fluorescent protein

Depositor

Natasha DeVore

Article

Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

Insert

Thermal Green Protein Q66E (TGP-E)

Tags

His tag

Expression

Bacterial

Promoter

lac operon

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA3C

Plasmid

#198576

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA3C (EBNA-3B/EBNA-3C Epstein-Barr virus)

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pM-mClover3

Plasmid

#228624

Purpose

Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mClover3

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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plexm-human LRRC33

Plasmid

#111600

Purpose

Express human LRRC33 in 393T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

LRRC33 (NRROS Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pR-CyOFP1

Plasmid

#228604

Purpose

Mammalian expression of CyOFP1, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

CyOFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D-5

Plasmid

#107866

Purpose

Bacterial expression of PIS, PI4K and PI4P5K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD and proD (in operon)

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA3B

Plasmid

#198574

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA3B (EBNA-3B/EBNA-3C Epstein-Barr virus)

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA3A

Plasmid

#198575

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA3A (EBNA-3A Epstein-Barr virus)

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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MT1E

Plasmid

#105702

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 1e

Depositor

Artur Krężel

Article

Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

Insert

Metallothionein 1e (MT1E Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-4

Plasmid

#228986

Purpose

For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-3

Plasmid

#228985

Purpose

For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-3

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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