We narrowed to 9,836 results for: pET

Showing: 2661 - 2680 of 9836 results

Results per page:

Sort results by:

pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

Plasmid

#241012

Purpose

VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-bla-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

Plasmid

#241011

Purpose

VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGF-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR82: pET-SUMO-USP21 (S93A/S113A/S115A)

Plasmid

#240190

Purpose

Bacterial expression for USP21 (S93A/S113A/S115A)

Depositor

Cynthia Wolberger

Insert

USP21 (USP21 Human)

Tags

6x-His-SUMO

Expression

Bacterial

Mutation

Changed Serine 93, 113, and 115 to Alanine

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-GST-TNRC6A-(935-984)-NES-mut

Plasmid

#42051

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

GST and His

Expression

Bacterial

Mutation

contain aa935-984, F951A, F955A, I958A, F960A

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

Plasmid

#209292

Purpose

Expresses R2Bm-Cas9(H840A) fusion in E. coli

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2

Tags

His, MBP, SpCas9(H840A), StrepII, and bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b(+)_rPD-L1nb

Plasmid

#226715

Purpose

Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag and Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-HIV p24

Plasmid

#212195

Purpose

Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

Depositor

Richard C. Willson

Insert

Chain A, Capsid protein p24, HIV type 1

Tags

Polyhistidine

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23a-H6-TEV-cSrc

Plasmid

#214233

Purpose

Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

Depositor

Neel Shah

Article

Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

Insert

cSrc Kinase domain (SRC Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28sumo-hRig-I DCARDS

Plasmid

#210828

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

Insert

hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)

Expression

Bacterial

Mutation

NA

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-C2m2-SNAP

Plasmid

#208773

Purpose

To express C2m2-SNAP in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Tags

SNAP-tag

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_mouse-pro-IL1B

Plasmid

#183386

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL1B (Il1b Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-CoilR-Cys

Plasmid

#181989

Purpose

Used to label proteins with the CoilE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

CoilR-Cys

Tags

His-Tag

Expression

Bacterial

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-miniR-DBCO

Plasmid

#181984

Purpose

Used to label proteins with the MiniE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniR-DBCO

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge