We narrowed to 9,957 results for: pET

Showing: 2661 - 2680 of 9957 results

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Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Publication

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Publication

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-PTBP1CCtoSS

Plasmid

#89157

Purpose

Recombinant expression of PTBP1 C250S,C251S in E.coli

Depositor

Frédéric Allain

Publication

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Publication

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Publication

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Tags

Myc

Expression

Bacterial

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only
pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET3a RegIIIg

Plasmid

#64936

Purpose

bacterial expression of processed mouse RegIIIgamma

Depositor

Lora Hooper

Publication

Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8.

Insert

RegIIIg (Reg3g Mouse)

Expression

Bacterial

Mutation

processed form

Promoter

T7

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
YPet-Lysosomes-20

Plasmid

#56636

Purpose

Localization: Lysosome Membrane, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LAMP1

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
YPet-LaminB1-10

Plasmid

#56635

Purpose

Localization: Nuclear Envelope, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LaminB1 (LMNB1 Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
YPet-Mito-7

Plasmid

#56637

Purpose

Localization: Mitochondria, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Publication

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET3a-AqAdk_Y52C

Plasmid

#18090

Depositor

Dorothee Kern

Publication

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad A. aeolicus)

Expression

Bacterial

Mutation

Tyr 52 changed to Cys

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
peT3a-AqAdk_MVGDH

Plasmid

#18092

Depositor

Dorothee Kern

Publication

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad A. aeolicus)

Tags

6xHis

Expression

Bacterial

Mutation

Tyr 52 changed to Cys, Val 145 changed to Cys, …

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
PET11a-Af2sir2

Plasmid

#8383

Depositor

Cynthia Wolberger

Publication

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Expression

Bacterial

Mutation

PET11a-Af2Sir2D103N

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET32b-MED19

Plasmid

#15438

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

His and Trx

Expression

Bacterial

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pETMutS-E41A

Plasmid

#13048

Depositor

Peggy Hsieh

Publication

Schofield et al J Biol Chem. 2001 Dec 7. 276(49):45505-8

Insert

MutS

Expression

Bacterial

Mutation

mutation Glu41 to Ala full-length

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

Plasmid

#85111

Purpose

expression of recombinant human mature (delta19) ETFalpha wt without tag

Depositor

Pal Falnes

Publication

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta19) ETFalpha (ETFA Human)

Expression

Bacterial

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1

Plasmid

#87737

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Publication

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

Plasmid

#223724

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Publication

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FIP200-CTR (RB1CC1 Human)

Tags

6xHis-TEV-EGFP

Expression

Bacterial

Mutation

aa 1429-1591 only

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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