We narrowed to 5,135 results for: codon optimized

1. pACYC-TtCas6-4xcrRNA4.5

   Plasmid

   #127764

   Purpose

   For expression of T. thermophilus Csm complex in E. coli (crRNA)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Synthetic CRISPR array containing four identical T. thermophilus spacers
   Cas6A
   

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

   Plasmid

   #71720

   Purpose

   Expressing an mRuby-tagged TIR1 in the soma of C. elegans

   Depositor

   Abby Dernburg

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
   

   Tags

   mRuby

   Expression

   Worm

   Mutation

   codon-optimized for expression in C. elegans; Two…

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBJG1 T7-8xHis-OGT

   Plasmid

   #154271

   Purpose

   Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   bacterial codon optimized

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_ancCas9d_Plasmid

   Plasmid

   #239444

   Purpose

   Expression plasmid codon-optimized for E. coli

   Depositor

   David Taylor

   Article

   Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

   Insert

   ancCas9d
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHA1.1-MFSD12-V5

   Plasmid

   #149508

   Purpose

   Stable expression of human MFSD12 with recoded nucleotide sequence.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   Codon Optimized Sequence

   Promoter

   Ubc

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a/Cas9-Cys

   Plasmid

   #53261

   Purpose

   Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

   Depositor

   Hyongbum Kim

   Article

   Ramakrishna et al Genome Res. 2014 Apr 2.

   Insert

   Cas9-Cys
   

   Tags

   6X His tag, HA epitope, and NLS

   Expression

   Bacterial

   Mutation

   Additional Cysteine is added to the C-terminal re…

   Promoter

   T7 Promoter

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSmart-EF1a-Nla-cas3

   Plasmid

   #178881

   Purpose

   Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas3c-bGH polyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cut190*_pET21b

   Plasmid

   #215404

   Purpose

   Expression construct for Cut190* gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Cut190*
   

   Expression

   Bacterial

   Mutation

   S226P/R228S

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKCcas9dO

   Plasmid

   #62552

   Purpose

   High efficiency Streptomyces genome editing by CRISPR/Cas9 system

   Depositor

   Weihong Jiang

   Article

   Huang et al Acta Biochim Biophys Sin (Shanghai). 201

   Inserts

   Scocas9
   sgRNA ()
   act-orf4 homology arm
   

   Use

   CRISPR; Bacterial genome editing

   Expression

   Bacterial

   Promoter

   j23119 and ptipA

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-YU2gp160

    Plasmid

    #100927

    Purpose

    Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (YU2) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDF0234 pCMV - huDisCas7-11 dead mutant

    Plasmid

    #172509

    Purpose

    Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pCMV - huDisCas7-11 dead mutant
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBF3060

    Plasmid

    #26853

    Purpose

    Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    GAL1

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.3-enCjCas9-BlastR

    Plasmid

    #220978

    Purpose

    Expresses human codon-optimized enCjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    enCjCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-MH6-EsCas13d

    Plasmid

    #108303

    Purpose

    Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    EsCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-Q842gp160

    Plasmid

    #100931

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZS157 CRISPEY RT/Cas9

    Plasmid

    #114454

    Purpose

    Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Insert

    SpCas9, Ec86-RT
    

    Use

    CRISPR

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    S. cerevisiae codon optimized

    Promoter

    GAL1-GAL10

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-MH6-RspCas13d

    Plasmid

    #108305

    Purpose

    Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    RspCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HDM_RSV_A_1982_F

    Plasmid

    #237355

    Purpose

    Expression plasmid for RSV A F from 1982

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 1982 human codon optimized from Genbank Accesion KJ723472
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. HDM_RSV_A_2020_F

    Plasmid

    #237356

    Purpose

    Expression plasmid for RSV A F from 2020

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 2020 human codon optimized from Genbank Accesion PP495954
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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