We narrowed to 6,648 results for: hwa

1. YF-Cdc42(CA)

   Plasmid

   #20152

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   YFP-FKBP-Cdc42(CA) (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cdc42H was inserted into the multiple cloning sit…

   Promoter

   Available Since

   March 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-FLAG-Sestrin3

   Plasmid

   #72592

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN3 (SESN3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCMV5 MEKK2 WT

   Plasmid

   #12182

   Depositor

   Gary Johnson

   Article

   Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

   Insert

   MEKK2 (Map3k2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-flag Mst2 (KR)

   Plasmid

   #27372

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   Mst2 (STK3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   K56R (catalytically inactive)

   Promoter

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pLXSN-H-Ras_N17

   Plasmid

   #39537

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS N17 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N17

   Promoter

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCMV5 MEKK4alpha WT

   Plasmid

   #12187

   Depositor

   Gary Johnson

   Article

   Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

   Insert

   MEKK4 (Map3k4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pRK5-Flag CASTOR1 I280A

   Plasmid

   #84495

   Purpose

   overexpression of CASTOR1 I280A mutant

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 I280A (GATSL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I280A

   Promoter

   CMV

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

8. SYMPK

   Plasmid

   #156071

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SYMPK (SYMPK Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNAIIIB cdc42 QL

    Plasmid

    #61636

    Purpose

    mammalian expression of cdc42 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    UBQ10

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET23-PBD(65-150)-N-Cys

    Plasmid

    #13722

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    PBD(65-150)-N-Cys (PAK1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Met-Cys-Gly-PBD(65-150)-

    Promoter

    Available Since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pTET2-RlucCP

    Plasmid

    #198352

    Purpose

    Part of Firefly reporter system, encodes Renilla luciferase

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTET2

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRPF18

    Plasmid

    #155846

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF18 (PRPF18 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWZL Hygro-NFLAG TRF1 deltaA deltaM

    Plasmid

    #16297

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted acidic domain (aa1-66) and myb domain (aa…

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

16. THOC3

    Plasmid

    #156082

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    THOC3 (THOC3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1 kb Axin2 basic

    Plasmid

    #25701

    Depositor

    Eric Fearon

    Article

    Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

    Insert

    Axin 2 promoter (AXIN2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pEF005-sgRNA-shuffle-in

    Plasmid

    #104441

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    U6 promoter

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIMVAd40R

    Plasmid

    #98309

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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