We narrowed to 6,235 results for: nls

1. pET28a-EGFP-CNA35

   Plasmid

   #61603

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with EGFP

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   EGFP and CNA35
   

   Tags

   His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEMS-II-dCas13-APO1-YTH

   Plasmid

   #204767

   Purpose

   GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   dCas13-NLS-APO1-YTH-HA
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   Ef1alpha

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pnCas9-PBE

   Plasmid

   #98164

   Purpose

   Expresses CRISPR base editor in protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Expression

   Plant

   Promoter

   Ubi

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-mCherry-CNA35

   Plasmid

   #61607

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mCherry

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mCherry and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAS

    Plasmid

    #206994

    Purpose

    Plasmid expressing both SpCas9 and SaCas9

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Insert

    SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )
    

    Tags

    P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dCas13d-BFP

    Plasmid

    #215720

    Purpose

    Catalytically inactive dCasRx with 2A-BFP

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-hAsCpf1(TATV) (pY220)

    Plasmid

    #89353

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TATV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CMV

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSmart-EF1a-Nla-cas3

    Plasmid

    #178881

    Purpose

    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas3c-bGH polyA
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCR1276

    Plasmid

    #111115

    Purpose

    Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51C gRNA (RAD51C Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR1275

    Plasmid

    #111114

    Purpose

    Expresses RAD51C_+_56770199.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51C gRNA (RAD51C Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH-nCas9-PBE

    Plasmid

    #98163

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Rice

    Expression

    Plant

    Promoter

    Ubi

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge