We narrowed to 4,052 results for: gast

1. pWC2

   Plasmid

   #15960

   Depositor

   Jeff Gelles

   Article

   Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

   Insert

   Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
   

   Use

   Tags

   BCCP and His6

   Expression

   Bacterial

   Mutation

   amino acids 1-401

   Promoter

   Available since

   Nov. 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FLAG-MED17

   Plasmid

   #15410

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

   Insert

   MED17 (MED17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKC31

   Plasmid

   #226777

   Purpose

   Expresses Drosophila UNC-104(R562H) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (R562H) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Arginine 562 to Histidine (bris mutation)

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

   Plasmid

   #163928

   Purpose

   Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p026 pCS2-EnR

   Plasmid

   #11028

   Depositor

   Ramesh Shivdasani

   Article

   Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

   Insert

   engrailed (en Fly)
   

   Use

   Xenopus, mammalian, avian, and zebrafish

   Tags

   Expression

   Mutation

   Engrailed repressor domain (bases encoding amino …

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBP-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199202

   Purpose

   Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein-Gal4[21-881]-C-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KHBD00532

   Plasmid

   #39630

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3629 (Dll Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pMM627

   Plasmid

   #112532

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   luxCDABE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23107, PL(HrtO), and ProD

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KHBD00188

    Plasmid

    #34340

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1034 (bcd Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3_NSnoFbox-vhhGFP4

    Plasmid

    #35580

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSnoFbox-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F box domain of slmb deleted.

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Myc-Bnip3FL

    Plasmid

    #100796

    Purpose

    Mammalian expression of Bnip3 full length splice variant

    Depositor

    Joseph Gordon

    Article

    Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

    Insert

    mus Bnip3 (Bnip3 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid

    #163917

    Purpose

    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUASp YFP Ras85D CA

    Plasmid

    #52247

    Purpose

    Drosophila expression of YFP-Ras85D constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMT-HuC:Dendra2

    Plasmid

    #80904

    Purpose

    Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

    Insert

    Dendra2 (elavl3 Synthetic)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    HuC

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217513

    Purpose

    Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 908H

    Plasmid

    #160240

    Purpose

    TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Tet Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge