We narrowed to 7,359 results for: ALP

1. ATP6 alphaDdCBE T1

   Plasmid

   #221960

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP6 alphaDdCBE T2

   Plasmid

   #221961

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD(Gib)_5'alpha

   Plasmid

   #223863

   Purpose

   4G-cloning donor for 5'-Gibson overhang, alpha-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   5'alpha sequence and spacer
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pscALPSpuro-MDA5delta2CARD

   Plasmid

   #174225

   Purpose

   Expresses human MDA5 that lacks 2 CARD domains

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   MDA5 (IFIH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   deletion of 2CARD

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Palpha6 and Interface-4 (a)

   Plasmid

   #204434

   Purpose

   Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

   Depositor

   Keir Neuman

   Article

   Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

   Insert

   hOpa1 isoform 1 (OPA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   F860A Y861A Y862A Y863A Q864A R865A H866A F867A

   Promoter

   CMV

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MT2A_alpha-domain

   Plasmid

   #200302

   Purpose

   Bacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2a

   Depositor

   Artur Krężel

   Article

   Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

   Insert

   alpha-domain of metallothionein 2a (MT2A Human)
   

   Use

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_SNIPR_CD8alpha variant 3

   Plasmid

   #188363

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant3-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-SpGalpha-i

   Plasmid

   #185568

   Purpose

   to see protein dynamics

   Depositor

   Mamiko Yajima

   Article

   Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

   Insert

   Galpha-i
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   GFP, S-Tag

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-nE-mHP1alpha

   Plasmid

   #181895

   Purpose

   Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   nE-mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. P[acman]-Alpha1

    Plasmid

    #165858

    Purpose

    Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pR6Kg-Alpha2

    Plasmid

    #165863

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_GNAT2_G-alpha

    Plasmid

    #109947

    Purpose

    Protein expression and purification of GNAT2_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAT2_G-alpha (GNAT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_GGA3_Alpha-adaptinC2

    Plasmid

    #109926

    Purpose

    Protein expression and purification of GGA3_Alpha-adaptinC2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GGA3_Alpha-adaptinC2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-AlpJ

    Plasmid

    #139598

    Purpose

    expresses AlpJ in E. coli

    Depositor

    Emily Balskus

    Article

    Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

    Insert

    alpJ
    

    Use

    Tags

    N terminal hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ptrc::galP_NS1

    Plasmid

    #101843

    Purpose

    drives expression of galP transporter from NS1 locus

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    Ptrc promoter driving GalP transporter expression
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB1alpha1_SF (GB0106)

    Plasmid

    #68232

    Purpose

    Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScalps_puro_mIL-2Rα ST

    Plasmid

    #59919

    Purpose

    Expresses mouse interleukin-2 receptor alpha chain mutated

    Depositor

    Silvia Monticelli

    Article

    Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

    Insert

    interleukin 2 receptor alpha (Il2ra Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed sequence of the intracellular (C-terminal…

    Promoter

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET16b-alpha3D

    Plasmid

    #53310

    Purpose

    bacterial expression of alpha3D

    Depositor

    William DeGrado

    Article

    Walsh et al Proc Natl Acad Sci U S A. 1999 May 11;96

    Insert

    alpha3D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

20. TRalpha shRNA #1

    Plasmid

    #32619

    Depositor

    Connie Cepko

    Article

    Billings et al PLoS One. 2010 . 5(10):e13739.

    Insert

    TRalpha shRNA #1 (THRA Chicken)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only