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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 398 results for: Vibrio

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Showing: 261 - 280 of 398 results

  2. pSL0828 (pQCascade_crRNA-4)

    Plasmid
    #130637
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130637-plasmid-map-sequence-id-255021

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  3. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid
    #130640
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on TniQ
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130640-plasmid-map-sequence-id-255648

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  9. pQdCas9-sgempty

    Plasmid
    #159523
    Purpose
    Expression of dCas9 driven by lux cassette from Vibrio fischeri
    Depositor
    Radhakrishnan Mahadevan
    Publication
    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    159523-plasmid-map-sequence-id-315103

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  11. NT-CRISPR plasmid collection

    Plasmid kit
    #1000000189
    Purpose
    Plasmids for genome editing in Vibrio natriegens
    Depositor
    Anke Becker
    Application
    Genome Editing
    Vector type
    Bacterial Expression
    Editing type
    CRISPR
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  17. pSL0762 (pQCascade_crRNA-nt)

    Plasmid
    #130636
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130636-plasmid-map-sequence-id-255017

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  18. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Publication
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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  19. pBFC0619

    Plasmid
    #186690
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Publication
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    186690-plasmid-map-sequence-id-373180

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Showing: 261 - 280 of 398 results