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pUNC2001

Plasmid

#67280

Purpose

Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw

Depositor

Aziz Sancar

Article

Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54

Insert

Vibrio cholera photolyase

Tags

maltose binding protein

Expression

Bacterial

Mutation

none known

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pWSK29-wzzO139

Plasmid

#172970

Purpose

pWSK29 with Vibrio cholerae wzzO139 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzz gene from Vibrio cholerae O139

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPM1001

Plasmid

#167548

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Inaba serotype

Use

Cosmid

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Vibrio_vulnificus

Plasmid

#224405

Purpose

For expression of the type I CBASS system encoded by Vibrio vulnificus

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Endonuclease_Phospholipase_VvCdnG_AGS-C

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Vibrio_campbellii

Plasmid

#224404

Purpose

For expression of the type I CBASS system encoded by Vibrio campbellii

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Endonuclease_VcCdnG_AGS-C

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Gal tr-lux

Plasmid

#1330

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

Bacterial luciferase-V.h.CTP5

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Gal ts-lux

Plasmid

#1329

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

bacterial luciferase-V.h.MAV

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-pLtetO-gfp

Plasmid

#89476

Purpose

Broad-host plasmid expressing gfp under pLtetO promoter

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pLtetO-GFPmut3b

Promoter

pLtetO

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pST_140_LVL2 cam

Plasmid

#179334

Purpose

NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Mutation

Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCM18

Plasmid

#78565

Purpose

60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

Depositor

James Kaper

Article

Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

Type

Empty backbone

Tags

luxCDABE

Expression

Bacterial

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSL0283 (pTnsABC)

Plasmid

#130633

Purpose

Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0527 (pDonor)

Plasmid

#130634

Purpose

Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (Mini-Tn)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCVD518

Plasmid

#66158

Purpose

Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

Depositor

James Kaper

Article

Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

Insert

tdh1

Use

Cloning vector

Promoter

Unknown

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
ePop

Plasmid

#50953

Purpose

Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

Depositor

Lingchong You

Article

Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

Inserts

E lysis protein
LuxI
LuxR

Mutation

Early stop codon introduced in mutated luxR resul…

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRaGE Pyl TAG GFP Y35TAG

Plasmid

#160041

Purpose

Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

Depositor

Lital Alfonta

Article

Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

Inserts

Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Mutation

Changed tyrosine 35 to TAG stop-codon (Site numbe…

Promoter

P70b promoter and T500 terminator, Vibrio natrieg…

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDL1403

Plasmid

#226635

Purpose

This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

Depositor

Andrew Camilli

Article

Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

Inserts

tfox
qstR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMMB66EH-aphA

Plasmid

#44392

Depositor

Victor DiRita

Article

Anthouard et al MBio. 2013 Aug 6;4(4). pii: e00403-13. d

Insert

AphA

Expression

Bacterial

Promoter

tac

Available Since

April 22, 2013

Availability

Academic Institutions and Nonprofits only

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