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Showing: 1 - 20 of 175 results

  4. pCVD518

    Plasmid
    #66158
    Purpose
    Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe
    Depositor
    James Kaper
    Article
    Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.
    Insert
    tdh1
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Availability
    Academic Institutions and Nonprofits only

  11. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  14. pCVD529

    Plasmid
    #66159
    Purpose
    A 2.7 kb HindIII fragment carrying the 2nd tdh gene copy was cloned from the chromosome of Vibrio parahemolyticus strain WP1 into the HindIII site of pBR322
    Depositor
    James Kaper
    Article
    Nishibuchi et al Mol Microbiol. 1990 Jan;4(1):87-99.
    Insert
    tdh2
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Availability
    Academic Institutions and Nonprofits only

  15. pCgVchcrRNA

    Plasmid
    #203815
    Purpose
    Expression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coli
    Depositor
    Sheng Yang
    Insert
    CRISPR(BsaI)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203815-plasmid-map-sequence-id-403361

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  16. pCgVchDonor

    Plasmid
    #203817
    Purpose
    DNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coli
    Depositor
    Sheng Yang
    Insert
    Mini-Tn, Spec cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203817-plasmid-map-sequence-id-403362

    Enlarge

  17. pBsVchCAST

    Plasmid
    #203812
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203812-plasmid-map-sequence-id-403828

    Enlarge

  18. pCgVchCAST-crtYf

    Plasmid
    #203816
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli
    Depositor
    Sheng Yang
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203816-plasmid-map-sequence-id-403367

    Enlarge

  19. pBsVchCAST-pyrE

    Plasmid
    #203813
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203813-plasmid-map-sequence-id-403827

    Enlarge

  20. pCVD531

    Plasmid
    #66160
    Purpose
    A 8.9 kb HindIII fragment carrying the tdh-like gene of Vibrio hollisae strain 9041 was cloned into the HindIII site of pBR322.
    Depositor
    James Kaper
    Article
    Nishibuchi et al FEMS Microbiol Lett. 1990 Feb;55(3):251-
    Insert
    tdh-like
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 175 results