We narrowed to 582 results for: ZAC

1. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

   Plasmid

   #216173

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDonor-PRF1-Exon3

   Plasmid

   #209075

   Purpose

   Targeting vector for the human PRF1 locus to replace exon 3 with repaired exon 3

   Depositor

   Klaus Rajewsky

   Article

   Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

   Insert

   PRF1-Exon3 HDRT (PRF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-BCR-ABL-p210-BT-FLAG

   Plasmid

   #205621

   Purpose

   Expression of BCR-ABL oncogene in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

   Insert

   BCR-ABL oncogene, p210 isoform b3a2
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   deleted BT domain

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti PGK mKate2-OGT WT (Puro)

   Plasmid

   #154290

   Purpose

   Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x FLAG and mKate2

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   PGK

   Available since

   Dec. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-BCR-ABL-p190-FLAG

   Plasmid

   #205620

   Purpose

   Expression of BCR-ABL oncogene in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

   Insert

   BCR-ABL oncogene, p190 isoform b3a2
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T117M- Please see depositor comments

   Promoter

   CMV

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

   Plasmid

   #216531

   Purpose

   Targeting vector for CRISPRd generation

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   ABI-dCas9-mCherry
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_023d-sgCiCh2-2

   Plasmid

   #202444

   Purpose

   Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   Chromosome 2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. YES1 Y537F

   Plasmid

   #51299

   Purpose

   Expression of YES1 Y537F

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YES1 (YES1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Y537F

   Promoter

   CMV

   Available since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2R3a-nosT

    Plasmid

    #71272

    Purpose

    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    nopaline synthase terminator
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TCF4-DN in pLX303

    Plasmid

    #42592

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    31 first amino acids of hTcf4 have been deleted

    Promoter

    CMV

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-HA-PQBP1

    Plasmid

    #25804

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Apr 21. ():.

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    6xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

13. p2R3a-3AT

    Plasmid

    #71273

    Purpose

    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR1A mKate2-hu_ncOGT K842M

    Plasmid

    #154284

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    K842M mutation (Catalytic Inactive)

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC8_MF-p10-mCh (VE5626)

    Plasmid

    #139772

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    mCherry fluorescent protein
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PH

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. USPL1cat-pETM30

    Plasmid

    #85768

    Purpose

    bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    T7-lac

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-FLAG-hEcto/Tif1g-CAmut

    Plasmid

    #20903

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

    Insert

    Ectodermin (TRIM33 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Point mutagenesis of C125 and C128 to Alanine (RI…

    Promoter

    Available since

    May 15, 2009

    Availability

    Academic Institutions and Nonprofits only

18. p2R3a-TagRFP-OcsT

    Plasmid

    #71270

    Purpose

    Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR1A mKate2-hu_ncOGT D554N

    Plasmid

    #154283

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    D554N mutation (Ser/Thr Glycosylation Impaired)

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. USPL1cat-pEYFP_C1

    Plasmid

    #85766

    Purpose

    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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