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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,121 results for: gibson assembly

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Showing: 261 - 280 of 1121 results

  2. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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  4. pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

    Plasmid
    #185682
    Purpose
    Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.
    Depositor
    Peter Walter
    Publication
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERN1 (ERN1 Synthetic, Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    K599A
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185682-plasmid-map-sequence-id-366403

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  14. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

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  15. pBTK601

    Plasmid
    #110607
    Purpose
    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)
    Depositor
    Jeffrey Barrick
    Publication
    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    Synthetic Biology
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    110607-plasmid-map-sequence-id-215544

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Showing: 261 - 280 of 1121 results