We narrowed to 1,106 results for: pFAST

1. pFastBacI-KDAC6 H611A

   Plasmid

   #224352

   Purpose

   Expresses human KDAC6 (HDAC6) H611A in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

   Insert

   KDAC6 (HDAC6 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Histidine 611 mutated to alanine

   Promoter

   Polyhedrin

   Available since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pFastBac-GST-TBK1

   Plasmid

   #221331

   Purpose

   TBK1 protein overexpression in Sf9 insect cells

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   TBK1 (Tbk1 Mouse)
   

   Use

   Tags

   6xHis-TEV-2xGST-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1-C17orf53-Strep

   Plasmid

   #211446

   Purpose

   generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-Tag

   Depositor

   Helmut Pospiech

   Insert

   C17orf53 (HROB Human)
   

   Use

   Tags

   Strep-Tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-C17orf53-FLAG

   Plasmid

   #211447

   Purpose

   generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-Tag

   Depositor

   Helmut Pospiech

   Insert

   C17orf53 (HROB Human)
   

   Use

   Tags

   FLAG-Tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1-bullfrog saxiphilin

   Plasmid

   #140595

   Purpose

   protein expression

   Depositor

   Dan Minor

   Article

   Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

   Insert

   saxiphilin bullfrog
   

   Use

   Tags

   GFP-His10

   Expression

   Insect

   Mutation

   Deletion of M20 - please see depositor comment

   Promoter

   polyhedrin promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac FAK W266A

   Plasmid

   #186144

   Purpose

   For expresseion of recombinant FAK using baccolovirus in SF9 cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PTK2 (PTK2 Mouse)
   

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   DNA mutation T796G;G797C Amino acid mutation W266A

   Promoter

   polyhedrin promoter

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG660 lyn11-pFAST

   Plasmid

   #172863

   Purpose

   expresses lyn11-pFAST in mammalian cells (inner membrane labeling)

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   Lyn11-pFAST
   

   Use

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1_GI.1_A37C-A44C

   Plasmid

   #162582

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Alanine 37 changed to Cysteine and Alanine 44 cha…

   Promoter

   polyhedring

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1_GI.1_A37I-A44L

   Plasmid

   #162583

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   A37I and A44L

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac1_GI.1_Q141V-P221L

    Plasmid

    #162584

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Glutamine 141 changed to Valine and Proline 221 c…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac1_GI.1_L144C-P221C

    Plasmid

    #162585

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Leucine 144 changed to Cysteine and Proline 221 c…

    Promoter

    polyhderin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1_GI.1_G131C-N172C

    Plasmid

    #162586

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Glycine 131 changed to Cysteine and Asparagine 17…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac1_GI.1_N167C-L169C

    Plasmid

    #162587

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Asparagine 167 changed to Cysteine and Leucine 16…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFast-dCry-V5His

    Plasmid

    #136240

    Purpose

    Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    DmCry
    

    Use

    Tags

    His and V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac-GST-Mps1-KD

    Plasmid

    #114066

    Purpose

    protein production

    Depositor

    Geert Kops

    Article

    Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

    Insert

    Mps1-KD (TTK Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Mps1 KD (D664A)

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-GST-Mps1-WT

    Plasmid

    #114065

    Purpose

    protein production

    Depositor

    Geert Kops

    Article

    Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

    Insert

    Mps1-WT (TTK Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    none

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Plekhm1 - C terminal pFastHFTb

    Plasmid

    #73829

    Purpose

    Express human Plekhm1 C-part in insect cells

    Depositor

    Paul Odgren

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Insect

    Mutation

    cloned C-terminal part of human Plekhm1 (aa 493- …

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

18. Plekhm1-N terminal pFastHFTb

    Plasmid

    #73828

    Purpose

    Express human Plekhm1 N-part in insect cells

    Depositor

    Paul Odgren

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Insect

    Mutation

    cloned N- terminal part of human Plekhm1 (aa 1- 4…

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pFastBac-Gm129-Flag

    Plasmid

    #53766

    Purpose

    baculovirus expression of Gm129

    Depositor

    Aziz Sancar

    Article

    Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

    Insert

    Gm129 (Ciart Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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