We narrowed to 9,933 results for: ada

1. sgRNA TR KO 2

   Plasmid

   #207610

   Purpose

   sgRNA 2 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   TCAGGCCGCAGGAAGAGGAA
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA TR KO 1

   Plasmid

   #207609

   Purpose

   sgRNA 1 to knockout TR by replacement with a PuroR cassette

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   ACCCTAACTGAGAAGGGCGT
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIwt-PRM-Wasabi

   Plasmid

   #195553

   Purpose

   Always-on expression of mWasabi reporter with transcription activation by wildtype CI

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   CIwt
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUCmini-NFKB1-PGK-EGFP

   Plasmid

   #204018

   Purpose

   Homologous donor template for NFKB1 knockout expressing EGFP

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUCmini-NFKB1-PGK-tdTomato

   Plasmid

   #204019

   Purpose

   Homologous donor template for NFKB1 knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUCmini-NFKB2-PGK-EGFP

   Plasmid

   #204020

   Purpose

   Homologous donor template for NFKB2 knockout expressing EGFP

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUCmini-NFKB2-PGK-tdTomato

   Plasmid

   #204021

   Purpose

   Homologous donor template for NFKB2 knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUPD2-HR1bR181A R185A-GFP-L (B3-B4)

   Plasmid

   #211857

   Purpose

   Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Mutation

   R181A R185A

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

   Plasmid

   #211863

   Purpose

   Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA

   Expression

   Mutation

   C492A

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBbA5k-AcrAB-AsLOV2*(543)

    Plasmid

    #210863

    Purpose

    IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

    Depositor

    Mary Dunlop

    Article

    Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

    Inserts

    acrA
    acrB
    

    Use

    Synthetic Biology

    Tags

    AsLOV2*(543)

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR2_IHFdist_rm

    Plasmid

    #162318

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHF distal site is removed

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. qTAG-C-sTagRFP-Zeo

    Plasmid

    #207734

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. qTAG-C-miRFP670nano3-Zeo

    Plasmid

    #207735

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. qTAG-N-Zeo-sTagRFP

    Plasmid

    #207701

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. qTAG-N-Zeo-miRFP670nano3

    Plasmid

    #207702

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. qTAG-C-sTagRFP-Blast

    Plasmid

    #207712

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. qTAG-N-Puro-sTagRFP

    Plasmid

    #207690

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. qTAG-N-Blast-sTagRFP

    Plasmid

    #207679

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCMV-PRLSS-yATOM_SH2-KDEL

    Plasmid

    #209714

    Purpose

    Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-yATOM_SH2-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-MBP-yATOM_WDR5 (R80A)

    Plasmid

    #209713

    Purpose

    Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    MBP-yATOM_WDR5 (R80A)
    

    Use

    Tags

    MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge