We narrowed to 5,570 results for: donor

1. REV human NANOS3 TALEN

   Plasmid

   #59723

   Purpose

   TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+)-FluoSTEP-JNKAR

   Plasmid

   #181857

   Purpose

   Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-JNKAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.180

   Plasmid

   #185001

   Purpose

   Express -Eco1 AAVS1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   AAVS1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HES7-NLuc-2A-tdTomato

   Plasmid

   #130932

   Purpose

   Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   HES7 (HES7 Human)
   

   Tags

   NanoLuc and tdTomato

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB2-hPER1-mScarlet-I-CD4-bla

   Plasmid

   #189980

   Purpose

   Donor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion protein

   Depositor

   Achim Kramer

   Article

   Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   mScarlet-I
   

   Use

   CRISPR

   Tags

   3xFlag

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PL652

   Plasmid

   #159456

   Purpose

   Donor empty vector with puromycin resistance gene expression cassette flanked by FRT

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYL236

   Plasmid

   #173184

   Purpose

   CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFETCh_KMT2B

   Plasmid

   #101070

   Purpose

   Donor vector for 3' FLAG tag of human KMT2B

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   KMT2B homology arms (KMT2B Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FASTHDR-Cterm-NanoLuc-Puromycin

    Plasmid

    #167209

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NanoLuc

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid

    #136405

    Purpose

    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    BbsI restriction site at position 437 was removed…

    Promoter

    U6, PDK

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CreLite

    Plasmid

    #131785

    Purpose

    AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX4

    Plasmid

    #183903

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti PUb

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX3

    Plasmid

    #183904

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti Polyubiquitin

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

    Plasmid

    #179428

    Purpose

    Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-(nospacer)-mch
    

    Expression

    Mammalian

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD_C2516b

    Plasmid

    #91085

    Purpose

    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    At7SL

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. KHBD00278

    Plasmid

    #34405

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9045 (Myb Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pORANGE Cacnb4-GFP KI

    Plasmid

    #139663

    Purpose

    Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFETCh_FOXM1_iso1

    Plasmid

    #135734

    Purpose

    Donor vector for 3' FLAG tag of human FOXM1_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    FOXM1_iso1 Homology arms (FOXM1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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