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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2781 - 2800 of 5523 results

  2. Tad-CBE(GCC3)

    Plasmid
    #235078
    Purpose
    Expresses Tad-CBE(GCC3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(GCC3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235078-plasmid-map-sequence-id-469792

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  4. Tad-CBE(GCG4)

    Plasmid
    #235080
    Purpose
    Expresses Tad-CBE(GCG4) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(GCG4)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235080-plasmid-map-sequence-id-466787

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  5. SpRY-Tad-CBE(ACC2)

    Plasmid
    #235081
    Purpose
    Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC2)-SpRY Cas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235081-plasmid-map-sequence-id-466818

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  7. Tad-CBE2.4

    Plasmid
    #235055
    Purpose
    Expresses Tad-CBE2.4 in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE2.4-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235055-plasmid-map-sequence-id-466794

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  8. Tad-CBE3.6

    Plasmid
    #235058
    Purpose
    Expresses Tad-CBE3.6 in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE3.6-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235058-plasmid-map-sequence-id-466807

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  9. Tad-CBE(ACA1)

    Plasmid
    #235059
    Purpose
    Expresses Tad-CBE(ACA1) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACA1)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235059-plasmid-map-sequence-id-466805

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  10. Tad-CBE(ACC3)

    Plasmid
    #235062
    Purpose
    Expresses Tad-CBE(ACC3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235062-plasmid-map-sequence-id-466793

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  20. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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Showing: 2781 - 2800 of 5523 results