We narrowed to 15,819 results for: pET

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-4H pET151

Plasmid

#159386

Purpose

Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Tags

his-tag, TEV cleavage site

Expression

Bacterial

Mutation

TLNRD1 4-helix domain (residues 143-273)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET30 W96A-GLTP

Plasmid

#170734

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

W96A-GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Mutation

W96A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Ec-iPGM-bio

Plasmid

#162575

Purpose

Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

E. coli iPGM bio-6His

Tags

6His tag, T7 tag, biotinylation sequence, and thr…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CVm-Sec22b

Plasmid

#168029

Purpose

Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Sec22b(A131-H190)-mVenus

Tags

Hisx6 and myc

Expression

Bacterial

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CVm-VAMP8

Plasmid

#168028

Purpose

Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP8(G7-R67)-mVenus

Tags

Hisx6 and myc

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head-LBD

Plasmid

#164837

Purpose

Expression of human talin2 head mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Mutation

Lipid binding deficient mutations

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET - FnR062.Y78V

Plasmid

#84801

Purpose

bacterial expression of FnR.0.6.2-Y78V protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78V

Tags

6xHis

Expression

Bacterial

Mutation

Y78V

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78A

Plasmid

#84784

Purpose

bacterial expression of FnR.0.6.2-Y78A protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78A

Tags

6xHis

Expression

Bacterial

Mutation

Y78A

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78C

Plasmid

#84785

Purpose

bacterial expression of FnR.0.6.2-Y78C protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78C

Tags

6xHis

Expression

Bacterial

Mutation

Y78C

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78E

Plasmid

#84787

Purpose

bacterial expression of FnR.0.6.2-Y78E protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78E

Tags

6xHis

Expression

Bacterial

Mutation

Y78E

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78L

Plasmid

#84793

Purpose

bacterial expression of FnR.0.6.2-Y78L protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78L

Tags

6xHis

Expression

Bacterial

Mutation

Y78L

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78M

Plasmid

#84794

Purpose

bacterial expression of FnR.0.6.2-Y78M protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78M

Tags

6xHis

Expression

Bacterial

Mutation

Y78M

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78N

Plasmid

#84795

Purpose

bacterial expression of FnR.0.6.2-Y78N protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78N

Tags

6xHis

Expression

Bacterial

Mutation

Y78N

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78Q

Plasmid

#84797

Purpose

bacterial expression of FnR.0.6.2-Y78Q protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78Q

Tags

6xHis

Expression

Bacterial

Mutation

Y78Q

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78T

Plasmid

#84800

Purpose

bacterial expression of FnR.0.6.2-Y78T protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78T

Tags

6xHis

Expression

Bacterial

Mutation

Y78T

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET-11a (D3 - L39V)

Plasmid

#136607

Purpose

Expresses DANCER-3 mutant (reversion L39V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (L39V reversion)

Tags

5x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I54V)

Plasmid

#136608

Purpose

Expresses DANCER-3 mutant (reversion I54V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I54V reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F34A)

Plasmid

#136606

Purpose

Expresses DANCER-3 mutant (reversion F34A)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F34A reversion)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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