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pTol2-HuC(elavl3)-JEDI3sub

Plasmid

#246621

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3sub under the HuC(elavl3) promoter for pan neuronal expression in zebrafish

Depositor

Francois St-Pierre

Publication

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub

Use

Zebrafish expression

Promoter

HuC(elavl3)

Available since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCitrine

Plasmid

#34951

Depositor

Amro Hamdoun

Publication

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
miR-206-1/miR-133b promoter GFP

Plasmid

#20793

Depositor

David Bartel

Publication

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-206-1/mir-133b (mir206-1 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
miR-124-5 promoter GFP

Plasmid

#20792

Depositor

David Bartel

Publication

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-124 (mir124-5 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

Plasmid

#200539

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

Plasmid

#200540

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

Plasmid

#200541

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA-FRT-acry:venus-pA-FRT

Plasmid

#200543

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry:venus-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA-FRT-acry:mCherry-pA-FRT

Plasmid

#200544

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry: mCherry -pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-1 without ATG

Plasmid

#200525

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-2 without ATG

Plasmid

#200526

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-mCherry-pA ORF-3 without ATG

Plasmid

#200527

Purpose

mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1

Plasmid

#200529

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2

Plasmid

#200530

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3

Plasmid

#200531

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-1 without ATG

Plasmid

#200534

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 1 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-2 without ATG

Plasmid

#200535

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 2 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_2a-BFP-pA ORF-3 without ATG

Plasmid

#200536

Purpose

mini circle middle entry plasmid for 2a-BFP-pA ORF 3 (without ATG) with flanking BsaI cut sites

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP

Use

Mini-golden

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-G-G mm

Plasmid

#221728

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-G-G mm

Use

Fish expression

Mutation

G-G mismatch of miR-430 site

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-10-11 mm

Plasmid

#221727

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

Depositor

David Bartel

Publication

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-10-11 mm

Use

Fish expression

Mutation

Mismatch at positions 10 and 11 of miR-430 site

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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