We narrowed to 8,512 results for: sgrna

1. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

   Plasmid

   #231367

   Purpose

   Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231368

   Purpose

   Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

   Plasmid

   #227773

   Purpose

   Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   Cas9-t2A-GFP
   4xU6:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 and tp1

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177360

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsine promoter and U6 promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177365

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-U6.3-sgRNA-non-targeting-control -GFP

   Plasmid

   #221844

   Purpose

   Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

   Depositor

   Stephen Terry

   Article

   O’Sullivan et al bioRxiv 2024.03.04.583298

   Inserts

   EGFP
   non-targeting control sgRNA- GCACTGCTACGATCTACACC
   

   Use

   CRISPR; Tol2 transposon optimised for chick expre…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GACG and U6.3 chick

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-EGFR-L858R-T790M-dual-nick sgRNA

   Plasmid

   #214101

   Purpose

   Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

   Plasmid

   #208111

   Purpose

   Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

   Insert

   SpG C, U6, scaffold
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CASI-InteinC-NG C aa714-1368-U6-Lmna sgRNA1

   Plasmid

   #206974

   Purpose

   Expresses NG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

   Insert

   NG aa714-1368, U6, Lmna sgRNA1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211692

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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