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pCRII-Topo Crim1 in situ probe

Plasmid

#45600

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Crim1 in situ probe (Crim1 Mouse)

Use

In situ

Mutation

insert contains bp#4062-4502 of NM_015800.3

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
CtkA_full_cmv-10

Plasmid

#27433

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

3xFLAG

Expression

Mammalian

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_300_cmv-10

Plasmid

#27434

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

3xFLAG

Expression

Mammalian

Mutation

residues 301-325 are truncated

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q/D179Q_cmv-10

Plasmid

#27435

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

3xFLAG

Expression

Mammalian

Mutation

D155Q/D179Q

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_full_pET21a

Plasmid

#27428

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_300_pET21a

Plasmid

#27429

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

residues 301-325 are truncated

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q_pET21a

Plasmid

#27430

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D155Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q/D179Q_pET21a

Plasmid

#27432

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D155Q/D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241137

Purpose

Expresses Anti-GCN4 scFv-sfGFP and HaloTag-tdMCP to track mature and nascent SunTag-tagged proteins and MS2 tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-GCN4 scFv
tdMCP

Tags

HaloTag and sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235297

Purpose

ComMAND open-loop circuit regulating mRuby2, CAG-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG (synthetic cytomegalovirus early enhancer + c…

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF4x4-bGH

Plasmid

#235295

Purpose

ComMAND closed-loop circuit regulating mRuby2 with 4 copies of microRNA target sites

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB3-ScVip1-186-522

Plasmid

#238311

Purpose

ScVip1-186-522 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-522

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB3-ScVip1-536-1107-H548A

Plasmid

#238319

Purpose

ScVip1-536-1107-H548A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H548A

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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H1x

Plasmid

#218111

Purpose

Encodes mus musculus histone 1 in Gateway entry vector

Depositor

Peter Hamilton

Article

Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

Insert

Histone H1x (H1f10 Mouse)

Use

Gateway cloning entry vector

Expression

Bacterial

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF5x1-mRuby2-miRE.FF5-bGH

Plasmid

#235259

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2-miRE.FF5-bGH

Plasmid

#235258

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB3-ScVip1-536-1107

Plasmid

#238312

Purpose

ScVip1-536-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge