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We narrowed to 1,492 results for: Neb

Showing: 281 - 300 of 1492 results

  1. pU6_(GLuc)_INT[3xK-T]

    Plasmid
    #68429
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three kink-turns
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68429-plasmid-map-sequence-id-125557

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  4. pU6_(GLuc)_INT[S1Apt]

    Plasmid
    #68425
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct with S1 streptavidin aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68425-plasmid-map-sequence-id-120203

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  5. pmalc4x_G252R_TEV

    Plasmid
    #47704
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with G252R mutation (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Glycine 252 Arginine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47704-plasmid-map-sequence-id-68124

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  6. pmalc4x_K398R_TEV

    Plasmid
    #47701
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with K398R mutation (MYOC Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Lysine 398 to Arginine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47701-plasmid-map-sequence-id-68116

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  8. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68417-plasmid-map-sequence-id-120195

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  14. plentiCRISPR-sgGOT1

    Plasmid
    #72874
    Purpose
    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    sgGOT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pGBW-m4092686

    Plasmid
    #153895
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nearly native encoding;inactivating mutations in …
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153895-plasmid-map-sequence-id-307318

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  18. pZH051

    Plasmid
    #47311
    Purpose
    Expresses Tsr-Venus-Ubiquitin-CI
    Depositor
    Jie Xiao
    Article
    Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-
    Insert
    wild type immunity region of lambda phage with tsr-venus-ub-cI
    Use
    Tags
    CI
    Expression
    Bacterial
    Mutation
    Promoter
    PR; PRM
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. Avr2-GFP pZK538

    Plasmid
    #118011
    Purpose
    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .
    Depositor
    FLW Takken
    Article
    et al Bio-protocol 8(24): e3114.
    Inserts
    dspAvr2
    mCherry
    Use
    Tags
    EGFP and HDEL
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    35S
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    118011-plasmid-map-sequence-id-307579

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Showing: 281 - 300 of 1492 results