We narrowed to 390 results for: SaCas9

1. pX601-mCherry

   Plasmid

   #84039

   Purpose

   Staphylococcus aureus (SaCas9) conjugated with mCherry

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Tags

   NLS and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpeCas9-HF

   Plasmid

   #192141

   Purpose

   Expresses highly specific SpeCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   SpeCas9-HF
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   SpeCas9-HF (R247A, N415A, S421A)

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

   Plasmid

   #113684

   Purpose

   EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   N/A
   

   Use

   AAV

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

   Plasmid

   #109318

   Purpose

   Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   SaCas9 RuvC gRNA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sha2Cas9-HF

   Plasmid

   #192142

   Purpose

   Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   Sha2Cas9-HF
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Sha2Cas9-HF (R247A and N415A)

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1029-AIDx-CP

   Plasmid

   #135341

   Purpose

   Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   CP 1029 nSpyCas9-hAIDx
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. S2 1058 ABEMax

   Plasmid

   #135356

   Purpose

   Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-ABEMax aa 1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. S2 1058 V82G ABEMax

   Plasmid

   #135361

   Purpose

   Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1058 nSpyCas9 V82G ABEMax
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AIDx-ID-202

    Plasmid

    #135344

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-202
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AIDx-ID-208

    Plasmid

    #135345

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-208
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AIDx-ID-468

    Plasmid

    #135346

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-468
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1010-AIDx-CP

    Plasmid

    #135340

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1010 nSpyCas9 -hAIDx
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1058-AIDx-CP

    Plasmid

    #135342

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1058 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

    Plasmid

    #89991

    Purpose

    Donor template for generation of SOX2-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PDG459 V3

    Plasmid

    #226958

    Purpose

    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PDG458 V3

    Plasmid

    #226959

    Purpose

    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458 V3

    Plasmid

    #226957

    Purpose

    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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