...aureus Cas9 (SaCas9, 1053 amino acids) displayed cleavage activity in mammalian cells. SaCas9 produced indels...vivo studies. Testing AAV-SaCas9 in vivo To test the efficiency of AAV-SaCas9 in vivo, the Zhang lab created...SpCas9, leading the group to focus their efforts on SaCas9 characterization for in vivo studies. One of the...To compare the off-target effects of SpCas9 and SaCas9, Zhang’s group used an approach called BLESS (direct...). Using this sensitive method, they found that SaCas9 did not display higher levels of off-target activity...created an all-in-one SaCas9 and sgRNA construct using the liver-specific serotype AAV8. Since the efficiency...compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome modification, but...

...from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single...61591 : PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592...single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons...61592 : PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression...for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids contain two ...two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a...

... SpCas9-VQR TadA-TadA* NGA 8 SaCas9 TadA-TadA* NNGRRT 9 SaCas9-KKH TadA-TadA* NNNRRT 8 SpCas9-...the SpCas9 with the mutant versions of SpCas9 and SaCas9, which recognize PAM sequences other than NGG (...Target-AID (PmCDA1) NGG 5 SpCas9-VQR rAPOBEC1 NGA 8 SaCas9-KKH rAPOBEC1 NNNRRT 8 ABE SpCas9 TadA-TadA* NGG...

...this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system is regulated... in Molecular Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA ...

...this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors for efficient...and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9. These nucleases should...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...

.... For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it...Campylobacter jejuni (CjCas9) is even smaller than SaCas9 and is also compatible with AAV delivery. NmCas9...Addgene Blog  Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page...

... tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

...Modified PAM specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with...

...by Kivanc Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target...footprint compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...

...CRISPR. AAV transduction Only compatible with SaCas9 (packaging limit ~4.5kb). CRISPR elements are inserted...AAV particles. Transient or stable expression of SaCas9 and/or gRNA. Infects dividing and non-dividing ...

...et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in ... by the fact that even small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all...

...length, NgAgo is shorter than SpCas9 (1368 aa), SaCas9 (1053 aa), or Cpf1 (1228 or 1307 aa depending on...

...like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter...

... SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  Acidaminococcus sp. (AsCpf1...

...photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility of this approach...This study generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs ...

...Features 85169  pJL-SaBE3  SaCas9 (NNGRRT)    85170  pJL-SaKKH-BE3  SaCas9 (NNGRRT)    85171  pBK-VQR-BE3...

...variants with alternative PAMs like NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting...

...recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library - Ampl133...

... only game in town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated...

...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...