We narrowed to 28 results for: SaCas9

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Learn about CRISPR plant base editors for plants

This blog series showcases some of the new CRISPR tools we have at Addgene. Read about CRISPR-Cas12b for human genome editing, plant base editors, string assembly gRNA cloning, lineage tracing tools, and more.

CRISPR Cas9 gRNA plasmids from the Zhang lab

Learn how Cpf1 stacks up against Cas9 and NgAgo

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP).

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.