...from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single...61591 : PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592...single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons...61592 : PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression...for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids contain two ...two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a...

...Modified PAM specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with...

... Staphylococcus aureus Cas9 (SaCas9) is ∼1 kb shorter than SpCas9, SaCas9 can be efficiently packaged ...packaged into AAV. Similar to SpCas9, the SaCas9 endonuclease is capable of modifying target genes in mammalian... Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing and non-dividing cells...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

...also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged...

...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...