... Staphylococcus aureus Cas9 (SaCas9) is ∼1 kb shorter than SpCas9, SaCas9 can be efficiently packaged ...packaged into AAV. Similar to SpCas9, the SaCas9 endonuclease is capable of modifying target genes in mammalian... Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing and non-dividing cells...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

...use in AAV, or containing Staphylococcus aureus (SaCas9) . AAV is also a promising method for gene therapy...