We narrowed to 21 results for: SaCas9

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Sept. 16, 2020, 1 p.m.

Learn about CRISPR plant base editors for plants

Jan. 3, 2019, 1:35 p.m.

This blog series showcases some of the new CRISPR tools we have at Addgene. Read about CRISPR-Cas12b for human genome editing, plant base editors, string assembly gRNA cloning, lineage tracing tools, and more.

March 21, 2019, 12:47 p.m.

Learn how Cpf1 stacks up against Cas9 and NgAgo

July 14, 2016, 2:30 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

March 17, 2020, 1:32 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

March 27, 2018, 1:32 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

June 9, 2016, 2:30 p.m.

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

Feb. 2, 2016, 5 p.m.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Sept. 3, 2020, 12:15 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

May 18, 2021, 1:15 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Feb. 4, 2025, 2:15 p.m.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Sept. 21, 2017, 2:07 p.m.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Oct. 14, 2015, 2:30 p.m.

Three studies recently showed that CRISPR can be used to treat Duchenne muscular dystrophy in mice. Learn about the work and obstacles to application.

Jan. 26, 2016, 3:30 p.m.