We narrowed to 421 results for: pet-28

#162667

pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162666

pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Feb. 3, 2021

Academic Institutions and Nonprofits only

#162668

pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162675

pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Feb. 3, 2021

Academic Institutions and Nonprofits only

#162679

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162684

pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162670

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162676

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Feb. 3, 2021

Academic Institutions and Nonprofits only

#162671

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162669

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162677

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 7, 2021

Academic Institutions and Nonprofits only

#162674

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.

Gregg Beckham

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Jan. 6, 2021

Academic Institutions and Nonprofits only