We narrowed to 5,570 results for: donor

1. pUC19/PLIN2 GFP-P2A-BFP

   Plasmid

   #202195

   Purpose

   knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP-P2A-BFP knock-in

   Promoter

   lac

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-iSAM

   Plasmid

   #211495

   Purpose

   AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

   Depositor

   Lesley Forrester, Antonella Fidanza

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   UniSAM insert
   TET-ON
   Neo Resistance
   Insulator
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-mCherry Tag

   Expression

   Mammalian

   Promoter

   EF1a, NA, Splicing acceptor, and TREGV promoter

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-23:TJP1-mEGFP

   Plasmid

   #87429

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MYH11-NanoLuc-2A-tdTomato

   Plasmid

   #132569

   Purpose

   Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

   Depositor

   James Thomson

   Article

   Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

   Insert

   Human MYH11 knockin homology arms (MYH11 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197065

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   I3-01
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197064

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-24:MYH10-mEGFP

   Plasmid

   #87428

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   MYH10 Homology Arms with mEGFP-linker (MYH10 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   May 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pORANGE Cplx1-GFP KI

   Plasmid

   #131475

   Purpose

   Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cplx1, Cplx1 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-pur-CAG-Bi-DREADD

   Plasmid

   #159457

   Purpose

   AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   Bi-DREADD
   

   Expression

   Mammalian

   Mutation

   fusion protein of hM3Dq-mCherry and HA-KORD seper…

   Promoter

   CAG

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. OCT4-eGFP-2A-Puro

    Plasmid

    #31939

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Inserts

    OCT4 (POU5F1 Human)
    eGFP (eGFP )
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB-hCRY1-mScarlet-I-CD4-bla

    Plasmid

    #179442

    Purpose

    Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mScarletI
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AICSDP-1:PXN-EGFP

    Plasmid

    #87420

    Purpose

    Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    PXN Homology Arms with linker-EGFP (PXN Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDONR-53BP1 1-1972 WT

    Plasmid

    #53446

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Mutation

    siRNA resistant and mutations A128V, T720A, A1347…

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDB-hPER2-Luciferase-hvTK-bla

    Plasmid

    #179450

    Purpose

    Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    Luciferase
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB-hPER2-mClover3-CD4-bla

    Plasmid

    #179448

    Purpose

    Donor vector to knock in mClover3 N-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mClover3
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-short_BrokenHeart (no BC)

    Plasmid

    #86950

    Purpose

    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    Brokenheart construct
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE GFP-RAB11a KI

    Plasmid

    #131499

    Purpose

    Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Rab11a, Rab11a Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDB-hPER2-mScarlet-I-CD4-bla

    Plasmid

    #179449

    Purpose

    Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mScarletI
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TV-hPITX3-forward

    Plasmid

    #22073

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3 (ASMD Human)
    

    Use

    Cre/Lox; Zfn donor

    Tags

    eGFP

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pbG-APRT-J-u32uni-PdTKmCh

    Plasmid

    #107279

    Purpose

    APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Inserts

    APRT (APRT Human)
    puro-deltaTK; CAG::mCherry
    

    Mutation

    c.402A>T, c.407T>C

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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