We narrowed to 6,482 results for: nls

Showing: 3021 - 3040 of 6482 results

Results per page:

Sort results by:

MSP2279

Plasmid

#70706

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A and H557A in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Intersectin I SH3 A domain (human)

Plasmid

#47413

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain A (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain A

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
VQR variant

Plasmid

#138560

Purpose

Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CFP-OSM

Plasmid

#12190

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pAT15546_nABE8e-SuperFi-Cas9

Plasmid

#184376

Purpose

Mammalian expression of SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-hNgAgo

Plasmid

#104384

Purpose

human codon optimized NgAgo; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. do

Insert

hNgAgo

Tags

3XFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIV494

Plasmid

#177701

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3a_026

Plasmid

#123778

Purpose

Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sadCAS9 (with NLS)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

stop at beginning of last exon

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_190-303

Plasmid

#118822

Purpose

Bacterial expression of hnRNPA2 truncation (residues 190 to 302).

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His tag and TEV

Expression

Bacterial

Mutation

contains amino acids 190-302

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT15544_nABE-SuperFi-Cas9

Plasmid

#184374

Purpose

Mammalian expression of SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

nABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
T7T3-PacI-elfn1_459to1199

Plasmid

#87671

Purpose

In situ probe for mouse Elfn1

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Elfn1 (Elfn1 Mouse)

Expression

Bacterial

Mutation

partial sequence, (459 to 1199bp off NM_175522.3)

Promoter

T7/T3

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X2-hDrp1(518-736)-Flag

Plasmid

#37244

Depositor

Christopher Counter, David Kashatus

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

Drp1 (DNM1L Human)

Tags

FLAG and GST

Expression

Bacterial

Mutation

contains AA 517-end

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only
Intersectin I SH3 E domain (human)

Plasmid

#47417

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

ITSN1 intersectin 1 (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain E

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pJ4M_hnRNPA2_FL_P298L

Plasmid

#139111

Purpose

Express FL hnRNPA2 P298L with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

P298L

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
kank3_pJet1.2

Plasmid

#121981

Purpose

for generating kank3 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank3 (kank3 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript-Maf

Plasmid

#98179

Purpose

Expression of a cMaf in situ hybridization probe

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

cMaf, partial mRNA (Maf Mouse)

Use

In situ hybridization

Promoter

T3/T7

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-eBE-S1

Plasmid

#101092

Purpose

Expresses eBE-S1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1505

Plasmid

#239830

Purpose

OrufIscB-REC mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge