We narrowed to 6,236 results for: nls

1. pTE3646

   Plasmid

   #137714

   Purpose

   Expresses LbCas12a RVRR variant in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   LbCas12a-RVRR
   

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK3258 - human expression plasmid for eSpCas9(1.1)

   Plasmid

   #101176

   Purpose

   Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K848A/K1003A/R1060A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. VanGlow-GL-DSCP

   Plasmid

   #83338

   Purpose

   for Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

   Depositor

   Cheng-Yu Lee

   Article

   Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   GFP::Luciferase(nls)

   Expression

   Insect

   Promoter

   Drosophila Synthetic Core Promoter

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MBP-MEK1 S222TAG

   Plasmid

   #68302

   Purpose

   N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 S222TAG (MAP2K1 Human, Synthetic)
   

   Tags

   His and MBP

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS1518

   Plasmid

   #205981

   Purpose

   FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS1514

   Plasmid

   #205977

   Purpose

   FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8/Cse1 from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS1515

   Plasmid

   #205978

   Purpose

   FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas11/Cse2 from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS1516

   Plasmid

   #205979

   Purpose

   FLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas7 from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MBP-MEK1 S218TAG

   Plasmid

   #68301

   Purpose

   N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)
   

   Tags

   His and MBP

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTHMT_hnRNPF_deltaPLD

    Plasmid

    #139115

    Purpose

    express FL hnRNPF without the prion like domain with N-terminal maltose binding protein tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPF (HNRNPF Human)
    

    Expression

    Bacterial

    Mutation

    delta365-415

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Promoter

    EF1a and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myopathy Loop Mutant Nuclear Myosin I YFP

    Plasmid

    #60243

    Purpose

    Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    Deleted I339, I340, G343, E344, E345,L346

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMKO shRNA Bad

    Plasmid

    #17237

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bad shRNA (BAD Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCMV dCas9-MC (eGFP)

    Plasmid

    #89931

    Purpose

    Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    dCas9-MC
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deactivated Cas9, fragment of M.SssI (residues 27…

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Intersectin I SH3 B domain (human)

    Plasmid

    #47414

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain B (ITSN1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain B

    Promoter

    tac

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pMM2-rPMCA3b

    Plasmid

    #47763

    Purpose

    mammalian expression of rat PMCA3b

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

    Insert

    PMCA3b (Atp2b3 Rat)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pMKO shRNA Bmf

    Plasmid

    #17236

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf shRNA (BMF Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

18. VRER variant

    Plasmid

    #138561

    Purpose

    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR1272

    Plasmid

    #111107

    Purpose

    Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCI gRNA (FANCI Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Intersectin I SH3 C domain (human)

    Plasmid

    #47415

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain C (ITSN1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain C

    Promoter

    tac

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only