We narrowed to 4,283 results for: biorxiv

1. pAAV-hSyn-GRAB_g5-HT2m

   Plasmid

   #208716

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT2m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-GRAB_g5-HT2m

   Plasmid

   #208710

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT2m in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT2m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-GRAB_r5-HTmut

   Plasmid

   #208714

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HTmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HTmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDisplay-GRAB_g5-HT3.0mut

   Plasmid

   #208712

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_EZH2gRNA15

   Plasmid

   #199587

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_EZH2gRNA16

   Plasmid

   #199588

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330_EZH2gRNA17

   Plasmid

   #199589

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330_EZH2gRNA14

   Plasmid

   #199586

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. OA-1067L

   Plasmid

   #200253

   Purpose

   pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

   Depositor

   Omar Akbari

   Article

   Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

   Insert

   6 gRNAs targeting Doublesex, Intersex, bTublin
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pminiT-mEmerald-MED1 donor

    Plasmid

    #197867

    Purpose

    Donor plasmid used for building mEmerald-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTij_sg_mmu_Med6_C_terminal

    Plasmid

    #197869

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pROS13_IDH2 #7

    Plasmid

    #166102

    Purpose

    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROS13_IDH1 #4

    Plasmid

    #166101

    Purpose

    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid

    #99692

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-AAV-CAG-flex-iGABASnFR2-WPRE

    Plasmid

    #218880

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiTet-P2A-mCherry BlastR

    Plasmid

    #186743

    Purpose

    Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    P2A-mcherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse b3 full length

    Plasmid

    #217814

    Purpose

    Full length mouse integrin b3 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB3 (Itgb3 Mouse)
    

    Use

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

    Plasmid

    #194278

    Purpose

    Expresses gRNA and dSaCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized dSaCas9 KRAB T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hUbC

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_rSERT_WT

    Plasmid

    #190743

    Purpose

    For expression of wild-type rat SERT in mammalian cells

    Depositor

    Gary Rudnick

    Article

    Singh et al bioRxiv 2022.06.13.495991

    Insert

    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    

    Use

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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