We narrowed to 4,097 results for: biorxiv

1. pDest-pol2-Smarcd1-Myc

   Plasmid

   #227716

   Purpose

   Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

   Depositor

   Kent Hunter

   Article

   Ross et al bioRxiv 2024.01.24.577061

   Insert

   Smarcd1 (Smarcd1 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   pol2

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL3346 PB-nicking guide-blast

   Plasmid

   #226665

   Purpose

   PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   protective nicking sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL435

   Plasmid

   #226628

   Purpose

   pcDNA-dTdT

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   catalytically deactivated TdT (DNTT Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   D343E, D345E

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-rTetR(SE)-KRAB-2A-mTurquoise

   Plasmid

   #225690

   Purpose

   Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-ZNF10 KRAB
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mTurquoise

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1302-AAV-EFSNC-dCjCas9-KRAB

   Plasmid

   #223147

   Purpose

   Expression of KRAB with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1312-AAV-EFSNC-dSaCas9-KRAB

   Plasmid

   #223157

   Purpose

   Expression of KRAB with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MBP-TEVpCS-ADGRG7 CTF-FLAG

   Plasmid

   #217705

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGR7 (ADGRG7 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-415

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-3xFLAG-SMAD1-active

   Plasmid

   #222579

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SMAD1 (SMAD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   S487E, S489E

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

   Plasmid

   #222990

   Purpose

   Bacterial expression of iGeoCas9(C1) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

    Plasmid

    #218870

    Purpose

    Mammalian expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    CAG

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-RSET-iGABASnFR2(no bind)-6xHis

    Plasmid

    #218887

    Purpose

    Bacterial expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F102G R168P

    Promoter

    T7

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid

    #216379

    Purpose

    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    6xHis and GP64 signal sequence

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

    Plasmid

    #207171

    Purpose

    expression plasmid for Gal4DBD-HIF-CAD.myc expression

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD Myc tag linker
    

    Tags

    2xMyc tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Duet_OPTN-thrombin-GST

    Plasmid

    #216843

    Purpose

    For expression in E. coli of OPTN (Internal ref. SMC1789)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    Optineurin (OPTN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac-Dual-GST-TEV-SINTBAD-mCherry

    Plasmid

    #208874

    Purpose

    For expression in insect cells of SINTBAD-mCherry

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Tags

    GST-TEV

    Expression

    Insect

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-MBP-TEVpCS-ADGRG4 CTF-FLAG

    Plasmid

    #217702

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG4 (ADGRG4 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2721

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-MBP-TEVpCS-ADGRF1 CTF-FLAG

    Plasmid

    #217694

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF1 (ADGRF1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-566

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MBP-TEVpCS-ADGRD2 CTF-FLAG

    Plasmid

    #217688

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRD2 (ADGRD2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-636

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-MBP-TEVpCS-ADGRD1 CTF-FLAG

    Plasmid

    #217687

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRD1 (ADGRD1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-544

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

    Plasmid

    #216404

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
    

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51, 56, Glutamine 70, 443 and Asp…

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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