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pAG415Gal_Gcn4 Aro2+

Plasmid

#232982

Purpose

Galactose iduced expression of Gcn4 Aro2+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+

Plasmid

#232983

Purpose

Galactose iduced expression of Gcn4 Aro3+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+GFP

Plasmid

#233003

Purpose

Galactose iduced expression of Gcn4 WW+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+GFP

Plasmid

#233001

Purpose

Galactose iduced expression of Gcn4 Aro2+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 S/ArotoL

Plasmid

#232979

Purpose

Galactose iduced expression of Gcn4 S/ArotoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED WHA

Plasmid

#232995

Purpose

Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidAHA

Plasmid

#232997

Purpose

Galactose iduced expression of Gcn4 9acidAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 9acidA

Plasmid

#232967

Purpose

Galactose iduced expression of Gcn4 9acidA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 9acidA

Tags

TEV cleavage site

Expression

Yeast

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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