We narrowed to 4,526 results for: gca

1. ROCK1 gRNA (BRDN0001146798)

   Plasmid

   #77595

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ROCK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ROCK1 (ROCK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA2_IL1RN

   Plasmid

   #64142

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET20b-delta133HtrA2 A141S

   Plasmid

   #16157

   Depositor

   L. Miguel Martins

   Article

   Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   A141S

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-SwitchON-sgCh2-2

   Plasmid

   #199637

   Purpose

   Tamoxifen-inducible expression of sgRNA control targeting intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1B POLE1_1

   Plasmid

   #160763

   Purpose

   Suppress POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLE1_1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-2-TGFBR2-m3_3'UTR

   Plasmid

   #31883

   Depositor

   Robert Blelloch

   Article

   Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

   Insert

   TGFBR2 mutant 3'UTR (TGFBR2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Third miR-302b, 372 7-mer seed match mutated from…

   Promoter

   SV40

   Available since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

7. PLK1 gRNA (BRDN0001144995)

   Plasmid

   #76459

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. gh266

   Plasmid

   #106865

   Purpose

   expression of gRNA targeting ALG12

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ALG12 (ALG12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. gh152

   Plasmid

   #106822

   Purpose

   expression of gRNA targeting GLT8D2

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   GLT8D2 (GLT8D2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1-puro-sgULK1-1

    Plasmid

    #109004

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ULK1-sgRNA #1 (ULK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PTK6 gRNA (BRDN0001149364)

    Plasmid

    #76490

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK6

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK6 (PTK6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pegAAT K342E correction

    Plasmid

    #169841

    Purpose

    Express a pegRNA used for correction (via A•T-to-G•C) of the E342K mutation

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    pegAAT K342E correction (SERPINA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

    Plasmid

    #162336

    Purpose

    Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6; mU6

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMP-DNMT1_2

    Plasmid

    #36383

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

15. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jean-Loup Faulon, Jerome Bonnet

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LRG-2.1T-neo-sgTAF12(human)#4.4

    Plasmid

    #105590

    Purpose

    lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting human TAF12 HFD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PIP5K1A gRNA (BRDN0001487080)

    Plasmid

    #77962

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL-p21UTRm2

    Plasmid

    #20879

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 2 (Position ~1100) G…

    Promoter

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

19. PRPS2 gRNA (BRDN0001147559)

    Plasmid

    #78005

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRPS2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRPS2 (PRPS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

    Plasmid

    #176838

    Purpose

    To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    sgPDX1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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