We narrowed to 9,206 results for: sgRNA

Showing: 3101 - 3120 of 9206 results

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pEPOR2KN0029

Plasmid

#117601

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0028

Plasmid

#117600

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0027

Plasmid

#117599

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_NbPDS(pEPOR1CB0114)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0026

Plasmid

#117598

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB021)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0025

Plasmid

#117597

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB020)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0024

Plasmid

#117596

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB019)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0023

Plasmid

#117595

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS+1 mismatch(pEPOR1CB0018)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0022

Plasmid

#117594

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_NbPDS(pEPOR1CB0114)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0050

Plasmid

#117617

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0051

Plasmid

#117618

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA2_ASCL1

Plasmid

#64130

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL2 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Publication

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

Plasmid

#138525

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

Depositor

Richard Jenner

Publication

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

Control non-PRC2 binding A-tract repeat RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Publication

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCFD5-NS

Plasmid

#149546

Purpose

Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Publication

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Publication

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCas12f1

Plasmid

#171182

Purpose

Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

Depositor

Kohsuke Honda

Publication

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

Cas12f1

Use

CRISPR

Expression

Bacterial

Promoter

cas9 promoter from Streptococcus pyogenes

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF1

Plasmid

#216537

Purpose

gRNA and template for MET 5'UTR uORF1 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Publication

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF1 mutation

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Publication

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL251 GG sgRNA

Plasmid

#126436

Purpose

To target a protospacer with GG at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GG at cut site

Expression

Mammalian

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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