Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 8,876 results for: ef1a;

Clear keywords
Showing: 3221 - 3240 of 8876 results

  1. JA1229

    Plasmid
    #49955
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-2 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. JA1217

    Plasmid
    #49954
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-1 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pLV-Blast_hCOL4A5

    Plasmid
    #198100
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198100-plasmid-map-sequence-id-389263

    Enlarge

  4. CMV-AausFP1-Z1

    Plasmid
    #135048
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    AausFP1
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135048-plasmid-map-sequence-id-269407

    Enlarge

  5. Cofilin1_pLVX-EF1-Cry2-mCherry

    Plasmid
    #64370
    Purpose
    lentiviral expression of Cofilin1 and Cry2-mCherry
    Depositor
    Matthieu Piel
    Insert
    Cofilin1 (CFL1 Human)
    Use
    Lentiviral
    Tags
    Cry2-mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. ELL3 Streptag

    Plasmid
    #127282
    Purpose
    Expresses mouse ELL3 with streptactin tag; does it degrade Myc?
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127282-plasmid-map-sequence-id-250028

    Enlarge

  7. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

    Enlarge

  8. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

    Enlarge

  10. gTBEv3

    Plasmid
    #220619
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220619-plasmid-map-sequence-id-436981

    Enlarge

  11. gTBEv4

    Plasmid
    #220620
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220620-plasmid-map-sequence-id-436970

    Enlarge

  12. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

    Enlarge

  13. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

    Enlarge

  15. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

    Enlarge

  16. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-482202

    Enlarge

  17. miniTurbo-dCasRx

    Plasmid
    #219821
    Purpose
    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCasRx
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219821-plasmid-map-sequence-id-440859

    Enlarge

  19. Aiolos in Artichoke

    Plasmid
    #74452
    Purpose
    Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    SFFV and EF1a
    Available Since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    74452-plasmid-map-sequence-id-140673

    Enlarge

  20. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

    Enlarge

Showing: 3221 - 3240 of 8876 results