We narrowed to 12,864 results for: sequence

1. TrpV1 scFv [N221/17]

   Plasmid

   #182082

   Purpose

   Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)
   

   Use

   Tags

   Sortase, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLY305

   Plasmid

   #184159

   Purpose

   Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   mutsfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   K140N, Mutations D10A and H840A on WT cas9. Synon…

   Promoter

   Pcon, PpspA-zraP-22, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-hAR-R617M,K618M,K632M,K633M

   Plasmid

   #115778

   Purpose

   Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

   Insert

   Androgen Receptor (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R617M, K618M ,K632M, K633M, insertion of Q81 and …

   Promoter

   CMV

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag‐mTim3 (del.Cyto)

   Plasmid

   #49209

   Purpose

   expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (del.Cyto) (Havcr2 Mouse)
   

   Use

   Tags

   CD8 signal sequence and FLAG

   Expression

   Mammalian

   Mutation

   cytosolic domain deleted, no Tim3 signal sequence…

   Promoter

   Ef1a

   Available since

   Dec. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. TrpC5 scFv [N67/15]

   Plasmid

   #190564

   Purpose

   Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL2-hOct4 DE-SV40-Luc

   Plasmid

   #26279

   Purpose

   Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

   Insert

   Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments below

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-TrpV3 [N15/39R]

   Plasmid

   #188210

   Purpose

   Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/39

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rufy3 scFv [N483/26]

   Plasmid

   #190558

   Purpose

   Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/26.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TrpV1 scFv [N221/17]

   Plasmid

   #190540

   Purpose

   Mammalian Expression of TrpV1 scFV. Derived from hybridoma N221/17.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   TrpV1 (Rattus norvegicus) recombinant scFV (Trpv1 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag‐mTim3 (T2 truncation)

    Plasmid

    #49211

    Purpose

    expresses Flag tagged mouse Tim3 (T2 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T2 truncation (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T2 truncation ( lacks all but one tyrosine, which…

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIR-31-Reporter

    Plasmid

    #71871

    Purpose

    Mammalian expression vector for the analysis of miR-31 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-31
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag‐mTim3 (T1 truncation)

    Plasmid

    #49210

    Purpose

    expresses Flag tagged mouse Tim3 (T1 truncation)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 T1 truncation (Havcr2 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    T1 truncation ( contains all but the three most C…

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. GP1BB

    Plasmid

    #51908

    Purpose

    Expresses full-length Platelet glycoprotein Ib beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GP1BB (GP1BB Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Stop Codon at amino acid 149, before CD4, bio and…

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-TDP-43 (PY-NLS)

    Plasmid

    #235490

    Purpose

    Expression of chimeric human TDP-43-EGFP bearing a PY-nuclear localization sequence (NLS) from HNRNPA1 in place of the native NLS sequence

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Replaced native NLS with PY-NLS from HNRNPA

    Promoter

    Available since

    May 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pRP.ABCA4 c.5461-10T

    Plasmid

    #226553

    Purpose

    Minigene encoding the ABCA4 c.5461-10T mutation

    Depositor

    Dave Wee

    Article

    Tommaso et al Molecular Therapy: Nucleic Acid (2025)

    Insert

    ABCA4 (ABCA4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.5461-10T intronic mutation

    Promoter

    CAG

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRP.ATM c.7865C>T

    Plasmid

    #226554

    Purpose

    Minigene encoding the ATM c.7865C>T mutation

    Depositor

    Dave Wee

    Article

    Tommaso et al Molecular Therapy: Nucleic Acid (2025)

    Insert

    ATM (ATM Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    c.7865C>T

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CROP-seq-copGFP-v2-no-scaffold

    Plasmid

    #228520

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CROP-seq-puro-v2-no-scaffold

    Plasmid

    #228521

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sg_Trp53_i4-ipUSEPR-TR657

    Plasmid

    #228930

    Purpose

    Knockdown of Trp53 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Trp53_i4 (Trp53 sequence: GTCGCTACCTACAGCCAGGA, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CROP-seq-mTurquoise2-v2-no-scaffold

    Plasmid

    #228522

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge