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LB601: pMVP/PB/eGFP-DEST

Plasmid

#121870

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pFastBac1 hBRM

Plasmid

#1956

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRM (SMARCA2 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Cdk6-DN-HA

Plasmid

#1869

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pCMV E2F1 E132

Plasmid

#24224

Purpose

Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

Depositor

Kristian Helin

Article

Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

Insert

E2F1 E132 (E2F1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pTAL-MajSat-FLAG-NLS-VP64

Plasmid

#69074

Purpose

Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Zeo-Sall4

Plasmid

#40797

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Sall4 (Sall4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pTAL-MajSat-FLAG-NLS-MCS

Plasmid

#69073

Purpose

Expresses TALE for mouse major satellite with FLAG tag under CAG promoter

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

TALE

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-HNF4α

Plasmid

#139305

Purpose

Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against HNF4α

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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HUNK_pCSdest

Plasmid

#53809

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HUNK (HUNK Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
HSPA13_pCSdest

Plasmid

#53872

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HSPA13 (HSPA13 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pSD057C

Plasmid

#218136

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36

Tags

rpoZ

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T_PV1_VP1

Plasmid

#202071

Purpose

Bacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

PV1 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro BRIT1

Plasmid

#16205

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

BRIT1 (MCPH1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only