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We narrowed to 4,955 results for: codon optimized

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Showing: 3241 - 3260 of 4955 results

  1. pTFG002-ACE2(Mut)-HA

    Plasmid
    #191570
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.
    Depositor
    Joshua Leonard
    Publication
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human expression. Also contai…
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191570-plasmid-map-sequence-id-441411

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  2. pTFG001-ACE2(WT)-HA

    Plasmid
    #191569
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.
    Depositor
    Joshua Leonard
    Publication
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191569-plasmid-map-sequence-id-441362

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  3. HypaR-SpCas9

    Plasmid
    #126757
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126757-plasmid-map-sequence-id-412372

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  13. pSEVA251_ Ptrc.x.tetO2::D-HicDH

    Plasmid
    #185456
    Purpose
    CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.
    Depositor
    Wolfgang Kroutil
    Publication
    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.
    Insert
    D-HicDH from Lactobacillus paracasei DSM 20008
    Expression
    Bacterial
    Promoter
    Ptrc.x.tetO2
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185456-plasmid-map-sequence-id-367933

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  14. pRWJB006

    Plasmid
    #160113
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free
    Depositor
    Detlef Weigel
    Publication
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    35S
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160113-plasmid-map-sequence-id-325470

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  15. pRWJB004

    Plasmid
    #160112
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Publication
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    UBQ10
    Available since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160112-plasmid-map-sequence-id-325469

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  16. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139096
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139096-plasmid-map-sequence-id-273894

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  17. pBT3038

    Plasmid
    #122545
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_glo-1::CemOrange2 (glo-1 Nematode, Synthetic)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122545-plasmid-map-sequence-id-238133

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  18. pCAGGS SARS-CoV-2 BQ.1.1 Spike

    Plasmid
    #193710
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike
    Depositor
    Marceline Côté
    Publication
    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 BQ.1.1 Spike (S )
    Expression
    Mammalian
    Mutation
    Contains the following mutations: T19I, LPPA24S, …
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Nov. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    193710-plasmid-map-sequence-id-377869

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  19. TadA-8e-eIscBn

    Plasmid
    #227018
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter
    Depositor
    Dali Li
    Publication
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227018-plasmid-map-sequence-id-453983

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  20. eIscB

    Plasmid
    #227016
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with extra SV40 NLS driven by CMV promoter
    Depositor
    Dali Li
    Publication
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    SV40 NLS-OgeuIscB-v3-SV40-nucleoplasmin NLS
    Mutation
    D96R/E84R/V159R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227016-plasmid-map-sequence-id-453979

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Showing: 3241 - 3260 of 4955 results