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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,620 results for: pET

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Showing: 3241 - 3260 of 15620 results

  3. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid
    #196975
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    6xHis and SNAP
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196975-plasmid-map-sequence-id-388920

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  7. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138207-plasmid-map-sequence-id-307410

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  14. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub(TA)-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84636-plasmid-map-sequence-id-176880

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  16. pET21a(+)_C.jejuni m23 peptidase

    Plasmid
    #83297
    Purpose
    Expresses m23 peptidase from Campylobacter jejuni
    Depositor
    SeWon Suh
    Insert
    ERS445056_01343
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    deleted amino acid 1-20
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83297-plasmid-map-sequence-id-164865

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  19. pET22-6His-SUMO-Nsp10

    Plasmid
    #154960
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154960-plasmid-map-sequence-id-306350

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Showing: 3241 - 3260 of 15620 results