We narrowed to 9,409 results for: try

1. pET21-pelB-LaG94-4

   Plasmid

   #228964

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-4
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaMIgG-5

   Plasmid

   #228991

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-5
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaTdT-8

   Plasmid

   #228976

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-8
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaTdT-9

   Plasmid

   #228977

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-9
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

   Plasmid

   #225649

   Purpose

   tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

   Depositor

   Andre Sanches Ribeiro

   Article

   Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

   Insert

   tetR/tetA, araBAD and LacO3O1 in tandem conformation
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   tetA, araBAD and LacO3O1 promoters

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM 1996

   Plasmid

   #217825

   Purpose

   Expresses putative photoreceptor SA-phr1 from S. alba. This gene shows high homology to the phr genes in prokaryotes.

   Depositor

   Aziz Sancar

   Article

   Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.

   Insert

   SA-phr1
   

   Use

   Tags

   Maltose-binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-GCaMP6f-WPRE

   Plasmid

   #216275

   Purpose

   AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   human Synapsin 1 (hSyn)

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-DDX5

   Plasmid

   #166163

   Purpose

   Gateway entry vector for N. furzeriDDX5

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221-mRuby3

   Plasmid

   #166136

   Purpose

   Gateway entry vector for mRuby3

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   mRuby3
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid

    #141237

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    Human elongation factor-1 alpha (EF-1 alpha)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2x3APWWP_K299E_D333A-IFP2.0C

    Plasmid

    #215753

    Purpose

    Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2x3APWWP_K299E_D333A-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1-Cdc45

    Plasmid

    #208796

    Purpose

    Xenopus laevis FLAG5-His10-TEV-Cdc45

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    Cdc45 (cdc45.L Frog)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-DONSON-WM

    Plasmid

    #208798

    Purpose

    Xenopus laevis His6-Myc-DONSON (W234L, M463T)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    W234L; M463T

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-DONSON-S437A

    Plasmid

    #208803

    Purpose

    Xenopus laevis His6-Myc-DONSON (S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    S437A

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-DONSON-ΔD1

    Plasmid

    #208804

    Purpose

    Xenopus laevis His6-DONSON (1-155aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    ΔD1, deletion of 1-155aa

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GB1-His-TEV-A7 (2-180)

    Plasmid

    #190066

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GB1-His-TEV-A7-Strep (2-180)

    Plasmid

    #190067

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV and Strep

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR223-BBLF2/3

    Plasmid

    #195831

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BBLF2/3

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BBLF2/3 (BBLF2/BBLF3 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR223-BcRF1(vTBP)

    Plasmid

    #195832

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BcRF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BcRF1 (BcRF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR223-BCRF1(vIL10)

    Plasmid

    #195777

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BCRF1, vIL-10

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BCRF1, vIL-10 (BCRF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge