We narrowed to 9,747 results for: try

1. LA001: pMVP (L3-L2) P2A-mCherry + WPRE

   Plasmid

   #121774

   Purpose

   pMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-mCherry + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PMYT1_HUMAN_D0

   Plasmid

   #79687

   Purpose

   This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PMYT1
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. LA201: pMVP (L3-L2) P2A-eCFP + WPRE

   Plasmid

   #121776

   Purpose

   pMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-eCFP + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE ENTR CMV tdTomato L5-L2

   Plasmid

   #62152

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   tdTomato
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMuLE ENTR CMV PuroR L3-L2

   Plasmid

   #62163

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   Puromycin
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMuLE ENTR U6 n.s shRNA L5-L4

   Plasmid

   #62138

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   non-silencing (scrambled) shRNA
   

   Use

   RNAi; Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2

   Plasmid

   #62130

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   CRISPR; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMuLE ENTR CMV eGFP L5-L2

   Plasmid

   #62140

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   eGFP
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMuLE ENTR SV40 iRFP L3-L2

   Plasmid

   #62160

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and iRFP module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   iRFP
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-2B-Nkx2-5

    Plasmid

    #98612

    Purpose

    Gateway entry vector for NKX2-5

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b-His-TEV-SNAP-tag_cx

    Plasmid

    #167273

    Purpose

    Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    P179R

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-MyosinIIC-N-18

    Plasmid

    #54195

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

    Insert

    MyosinIIC
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pMuLE ENTR CMV eGFP R4-R3

    Plasmid

    #62141

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    eGFP
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMuLE ENTR U6 n.s shRNA L1-R5

    Plasmid

    #62135

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

    Plasmid

    #121828

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid

    #121806

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + EF1a::TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. KH001: pMVP (L3-L2) V5 epitope tag + WPRE

    Plasmid

    #121762

    Purpose

    pMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    V5 epitope tag + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-ARL3

    Plasmid

    #67320

    Purpose

    hArl3 with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMuLE ENTR CMV iRFP L1-R5

    Plasmid

    #62156

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    iRFP
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiGuide-Puro-CTRLg2 (CG510)

    Plasmid

    #139456

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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