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Plasmid#45938Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTags6xHis and Twin-StrepExpressionBacterialMutationPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only
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pTD-NTwinStrep_Sm
Plasmid#45937Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTags6xHis and Twin-StrepExpressionBacterialMutationPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMB.BIG1a.PRC2
Plasmid#234581PurposeExpression of EZH2, MBP-SUZ12, EED, RBBP4DepositorUseTagsExpressionInsectMutationPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMY.PRC2
Plasmid#234582PurposeExpression of ProtA-EZH2, SUZ12, EED and RBBP4DepositorUseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT17
Plasmid#223389PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT23
Plasmid#223395PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT26
Plasmid#223398PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT29
Plasmid#223401PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT14
Plasmid#223386PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUS
Plasmid#193865PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.DepositorInsertAtWUS E. Coli Codon Optimized
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 Ub-S297
Plasmid#169965PurposepDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsUbExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJR83
Plasmid#75028PurposemCherry^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmCherry^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized mCherryExpressionWormMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJR82
Plasmid#75027PurposeEGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertEGFP^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized EGFPExpressionWormMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgs2_Balbc
Plasmid#170301PurposeA knock-out vector for the mouse Ptgs2 in Balb/cDepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEP4 E02S CK2M EN2L
Plasmid#20924Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28DepositorArticleUseEpisomal/ebnaTagsExpressionMutationPromoterAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJYS2_crtYf
Plasmid#85544PurposeConstitutive transcription of crRNA of crtYf, Spectinomycin resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-T3G-CERP2
Plasmid#175498PurposeAll-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-T3G-ERP2
Plasmid#175188PurposeAll-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWS936
Plasmid#123050PurposeG_ subunit for creating C-terminal transplantsDepositorInsertGpa1 C-terminal Truncation Vector [3-4a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TA-ERN
Plasmid#80474PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only